Singh Maheshwari Kumari, Usha R, Hithayshree K R, Bindhu O S
Department of Biochemistry, Center for Post Graduate Studies, Jain University, 9th Main, 18th Cross, Jayanagar 3rd Block, Bangalore, Karnataka, India.
J Thromb Thrombolysis. 2015 Jan;39(1):43-9. doi: 10.1007/s11239-013-1012-y.
Pharmacological properties exhibited by latex of plants are due to various biologically active compounds including several proteolytic enzymes. Present study evaluates hemostatic potential of Tabernaemontana divaricata and Artocarpus altilis from Apocynaceae and Moraceae families respectively. The latex of these plants were initially subjected to dialysis and crude extracts were estimated for proteolytic activity using casein as the substrate. Mean caseinolytic activity for 100 μg of latex protein was found to be 56.16 ± 0.57 and 45 ± 0.3 U/h for T. divaricata and A. altilis respectively. Caseinolytic activity by both the plant extracts was higher than standard proteases, papain and trypsin. However the difference was significant (p < 0.05) with papain alone. Crude enzymes (CE) from both plants exhibited coagulant activity on human platelet poor plasma by recalcification time. A significant reduction in clotting time was exhibited by T. divaricata compared to A. altilis (p < 0.05). These results were further substantiated with fibrinogen agarose plate assay. Crude enzyme of both plants also hydrolyzed blood clot. Mean % of thrombolysis by T. divaricata was 80.75 ± 1.2 and that of A. altilis was 70.24 ± 1.52. Inhibition studies confirmed cysteine protease nature of CE. Comparative analysis revealed T. divaricata to be the best among the two for its hemostatic potential. This study scientifically validates the use of latex from these plants in the management of fresh cuts or wounds.
植物乳胶所展现出的药理特性归因于多种生物活性化合物，包括几种蛋白水解酶。本研究分别评估了夹竹桃科的狗牙花和桑科的面包树的止血潜力。这些植物的乳胶首先进行透析，然后以酪蛋白为底物估计粗提物的蛋白水解活性。对于100μg乳胶蛋白，狗牙花和面包树的平均酪蛋白水解活性分别为56.16±0.57和45±0.3U/h。两种植物提取物的酪蛋白水解活性均高于标准蛋白酶木瓜蛋白酶和胰蛋白酶。然而，仅与木瓜蛋白酶相比差异显著（p<0.05）。两种植物的粗酶（CE）通过复钙时间对人少血小板血浆表现出凝血活性。与面包树相比，狗牙花的凝血时间显著缩短（p<0.05）。这些结果通过纤维蛋白原琼脂糖平板试验得到进一步证实。两种植物的粗酶也能水解血凝块。狗牙花的平均溶栓率为80.75±1.2，面包树的平均溶栓率为70.24±1.52。抑制研究证实了粗酶的半胱氨酸蛋白酶性质。比较分析表明，狗牙花在止血潜力方面是两者中最好的。本研究科学地验证了这些植物的乳胶在处理新鲜切口或伤口中的应用。
