蓝舌病病毒非结构糖蛋白NS3的膜组织
Membrane organization of bluetongue virus nonstructural glycoprotein NS3.
作者信息
Bansal O B, Stokes A, Bansal A, Bishop D, Roy P
机构信息
Department of International Health, University of Alabama at Birmingham, 35294, USA.
出版信息
J Virol. 1998 Apr;72(4):3362-9. doi: 10.1128/JVI.72.4.3362-3369.1998.
Abstract
The smallest RNA segment (S10) of bluetongue virus (an orbivirus, family Reoviridae) encodes two closely related nonstructural proteins, the 229-amino-acid (aa) NS3 and the 216-aa NS3A. The proteins are found in glycosylated and nonglycosylated forms in infected cells (X. Wu, H. Iwata, S.-Y. Chen, R. W. Compans and P. Roy J. Virol. 66:7104-7112, 1992). The NS3/NS3A proteins have two hydrophobic domains (aa 118 to 141 and 162 to 182) and two potential asparagine-linked glycosylation sites (aa 63 and 150), one of which is located between the hydrophobic domains. To determine whether these features were used in the mature protein forms, we generated a series of mutants of the S10 gene and expressed them by using the vaccinia virus T7 polymerase transient-expression system. Our data indicate that both hydrophobic domains of NS3 span the cell membrane and that only the site at aa 150 is responsible for N-linked glycosylation of the NS3 proteins.
摘要
蓝舌病毒（呼肠孤病毒科环状病毒属）最小的RNA片段（S10）编码两种紧密相关的非结构蛋白，即229个氨基酸（aa）的NS3和216个氨基酸的NS3A。在受感染细胞中可发现这些蛋白的糖基化和非糖基化形式（X. Wu、H. Iwata、S.-Y. Chen、R. W. Compans和P. Roy，《病毒学杂志》66:7104 - 7112，1992年）。NS3/NS3A蛋白有两个疏水结构域（氨基酸118至141和162至182）以及两个潜在的天冬酰胺连接糖基化位点（氨基酸63和150），其中一个位于疏水结构域之间。为确定这些特征在成熟蛋白形式中是否有用，我们构建了一系列S10基因的突变体，并使用痘苗病毒T7聚合酶瞬时表达系统进行表达。我们的数据表明，NS3的两个疏水结构域均跨越细胞膜，并且只有氨基酸150处的位点负责NS3蛋白的N - 连接糖基化。
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本文引用的文献
1
Release of bluetongue virus-like particles from insect cells is mediated by BTV nonstructural protein NS3/NS3A.蓝舌病病毒样颗粒从昆虫细胞中的释放由蓝舌病病毒非结构蛋白NS3/NS3A介导。
Virology. 1993 Apr;193(2):592-603. doi: 10.1006/viro.1993.1167.
2
Glycosylation of the major outer capsid protein of bluetongue viruses.蓝舌病毒主要外 capsid 蛋白的糖基化作用。
Virology. 1993 May;194(1):350-4. doi: 10.1016/s0042-6822(83)90000-4.
3
Adaptation of bluetongue virus in mosquito cells results in overexpression of NS3 proteins and release of virus particles.蓝舌病毒在蚊细胞中的适应性导致NS3蛋白的过度表达和病毒颗粒的释放。
Arch Virol. 1995;140(5):967-74. doi: 10.1007/BF01314973.
4
Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus.高尔基体中与天冬酰胺连接的寡糖加工的区室化
J Cell Biol. 1983 Jul;97(1):270-5. doi: 10.1083/jcb.97.1.270.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.肽：N-糖苷酶F对天冬酰胺连接聚糖的去糖基化作用
Biochemistry. 1985 Aug 13;24(17):4665-71. doi: 10.1021/bi00338a028.
7
The compartmental organization of the Golgi apparatus.高尔基体的区室组织。
Sci Am. 1985 Sep;253(3):74-89. doi: 10.1038/scientificamerican0985-74.
8
Compartmental organization of the Golgi stack.高尔基体堆叠的区室化组织
Cell. 1985 Aug;42(1):13-21. doi: 10.1016/s0092-8674(85)80097-0.
9
Assembly of asparagine-linked oligosaccharides.天冬酰胺连接寡糖的组装
Annu Rev Biochem. 1985;54:631-64. doi: 10.1146/annurev.bi.54.070185.003215.
10
Biosynthesis of the v-sis gene product: signal sequence cleavage, glycosylation, and proteolytic processing.v-sis基因产物的生物合成：信号序列切割、糖基化和蛋白水解加工。
Mol Cell Biol. 1986 Apr;6(4):1343-8. doi: 10.1128/mcb.6.4.1343-1348.1986.
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