Romanenko E B, Demidenko Z N, Vanyushin B F
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899 Russia.
Biochemistry (Mosc). 1998 Feb;63(2):159-63.
It has been established that activities of RNA-polymerase, DNA-polymerase, DNA-methyltransferase, and sphingomyelinase in the liver nuclei isolated from newborn (2-day-old) rats are by 18, 25, 27, and 610%, respectively, higher than those in mature (6-month-old) rats. It has been also shown that the number of single-stranded stretches in rat liver nuclear DNA increases with age: it is about 2-fold higher in mature rats than in newborn rats. Thus, the postnatal period of ontogenesis is accompanied by both significant changes in DNA structure and decrease in activities of enzymes serving for the most important genetic processes in cells of animals.
已经确定，从新生（2日龄）大鼠分离的肝细胞核中RNA聚合酶、DNA聚合酶、DNA甲基转移酶和鞘磷脂酶的活性分别比成熟（6月龄）大鼠高18%、25%、27%和610%。还表明，大鼠肝细胞核DNA中单链片段的数量随年龄增加：成熟大鼠比新生大鼠高约2倍。因此，个体发育的出生后时期伴随着DNA结构的显著变化以及动物细胞中最重要遗传过程相关酶活性的降低。
