使用流式细胞术作为研究分离的活肝细胞中线粒体膜电位的工具。
Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes.
作者信息
Salvioli S, Maseroli R, Pazienza T L, Bobyleva V, Cossarizza A
机构信息
Department of Biomedical Sciences, Section of General Pathology, University of Modena, via Campi, 287, 41100 Modena, Italy.
出版信息
Biochemistry (Mosc). 1998 Feb;63(2):235-8.
The present paper describes the possibility of determination of mitochondrial membrane potential (Deltapsi) in isolated hepatocytes making use of a Deltapsi-sensitive dye, i.e., the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3, 3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and of cytofluorimetry. The validity of the method was proved by treating hepatocytes with FCCP (decrease of Deltapsi) and subsequent addition of 6-ketocholestanol (increase of Deltapsi). The results indicate that the proposed method may be used in laboratory practice.
本文描述了利用一种对线粒体膜电位（ΔΨ）敏感的染料，即亲脂性阳离子探针5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑羰花青碘化物（JC-1）和细胞荧光测定法，来测定分离的肝细胞中线粒体膜电位的可能性。用羰基氰化物间氯苯腙（FCCP）处理肝细胞（降低ΔΨ）并随后添加6-酮胆甾醇（增加ΔΨ），证明了该方法的有效性。结果表明，所提出的方法可用于实验室实践。
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