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辣椒红素 - 辣椒玉红素合成酶基因:控制辣椒红色果实颜色的y位点的候选基因。

The capsanthin-capsorubin synthase gene: a candidate gene for the y locus controlling the red fruit colour in pepper.

作者信息

Lefebvre V, Kuntz M, Camara B, Palloix A

机构信息

INRA, Station d'Amélioration des Plantes Maraîchères, Montfavet, France.

出版信息

Plant Mol Biol. 1998 Mar;36(5):785-9. doi: 10.1023/a:1005966313415.

DOI:10.1023/a:1005966313415
PMID:9526511
Abstract

The red colour of pepper fruits is determined by the y+ dominant allele and the yellow colour by the y recessive allele. The capsanthin-capsorubin synthase (CCS) gene is activated specifically during the final stages of pepper fruit ripening. RFLP and specific-PCR polymorphisms derived from the CCS gene were analysed in a F2 progeny of a red by yellow-fruited cross. They cosegregated completely with fruit colour. Our results support the hypothesis that the yellow phenotype might result from a deletion of the CCS gene. These specific markers were integrated into the genetic map and will be useful for marker assisted plant breeding.

摘要

辣椒果实的红色由显性等位基因y+决定,黄色由隐性等位基因y决定。辣椒红素 - 辣椒玉红素合酶(CCS)基因在辣椒果实成熟的最后阶段被特异性激活。在一个红色果实与黄色果实杂交的F2后代中分析了源自CCS基因的限制性片段长度多态性(RFLP)和特异性聚合酶链反应(PCR)多态性。它们与果实颜色完全共分离。我们的结果支持这样的假设,即黄色表型可能是由于CCS基因的缺失导致的。这些特异性标记已整合到遗传图谱中,将有助于标记辅助植物育种。

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本文引用的文献

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Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies.利用分子标记和双单倍体后代构建辣椒种内整合连锁图谱。
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Developmental and stress regulation of gene expression for plastid and cytosolic isoprenoid pathways in pepper fruits.辣椒果实中质体和胞质类异戊二烯途径基因表达的发育调控与胁迫调控
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Biochemistry and molecular biology of chromoplast development.
多组学分析为深入了解[具体物种]果实颜色形成的潜在机制提供了线索。 (注:原文中“in”后面缺少具体内容)
Front Plant Sci. 2024 Nov 6;15:1448060. doi: 10.3389/fpls.2024.1448060. eCollection 2024.
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Mapping and cloning of pepper fruit color-related genes based on BSA-seq technology.基于BSA-seq技术的辣椒果实颜色相关基因的定位与克隆
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Genome-Wide Analysis of Fruit Color and Carotenoid Content in Core Collection.核心种质库中果实颜色和类胡萝卜素含量的全基因组分析。
Plants (Basel). 2024 Sep 12;13(18):2562. doi: 10.3390/plants13182562.
6
Cloning and Analysis of Expression of Genes Related to Carotenoid Metabolism in Different Fruit Color Mutants of Pepper ( L.).辣椒不同果实颜色突变体中与类胡萝卜素代谢相关基因的克隆与表达分析。
Genes (Basel). 2024 Feb 28;15(3):315. doi: 10.3390/genes15030315.
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Quantitative PCR.
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Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid.质体中叶黄素的生物合成:催化5,6-环氧类胡萝卜素转化为酮类胡萝卜素的一种酶的分离与分子克隆
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AFLP: a new technique for DNA fingerprinting.扩增片段长度多态性:一种用于DNA指纹分析的新技术。
Nucleic Acids Res. 1995 Nov 11;23(21):4407-14. doi: 10.1093/nar/23.21.4407.
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MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.MAPMAKER:一个用于构建实验群体和自然群体初级遗传连锁图谱的交互式计算机程序包。
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Fingerprinting genomes using PCR with arbitrary primers.使用任意引物通过聚合酶链反应对基因组进行指纹分析。
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.用任意引物扩增的DNA多态性可作为遗传标记。
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