Hambartsoumian E
Department of Obstetrics and Gynecology, Antoine Beclere Hospital, Clamart, France.
Gynecol Endocrinol. 1998 Feb;12(1):17-22. doi: 10.3109/09513599809024965.
The expression of leukemia inhibitory factor (LIF) by murine uterus was shown to be regulated by maternal hormones and was not dependent on the presence of an embryo. The objective of this study was to investigate whether in humans the secretion of LIF during early pregnancy is under maternal control and whether its production is correlated with pregnancy hormones, progesterone and beta-human chorionic gonadotropin (beta hCG). To exclude the possibility of paracrine interaction of decidua with trophoblast, we examined the secretion of LIF in women with extrauterine pregnancy. The present study was designed as a prospective, blinded, clinical and immunobiochemical study. The endometrial biopsies were performed on 12 women during surgery for ectopic pregnancy. On the same day, the level of progesterone and beta hCG in maternal plasma was examined. LIF concentration was determined in supernatants taken from cultured decidual explants. LIF production by decidual culture explants was found in all women with an ectopic pregnancy (Median 5015 pg, range 1389-19,304 pg). There was no correlation between the LIF production and the term of pregnancy, or with the level of circulated beta hCG (p > 0.05). However, when the concentration of progesterone in circulating plasma was less than 5 ng/ml, the secretion of LIF was 2.3-fold higher as compared to women who had progesterone levels of more than 5 ng/ml (p < 0.01). Therefore, we conclude that LIF is actively produced by human decidua and that the production of this cytokine does not depend on the presence of fetotrophoblast. This study demonstrates for the first time that progesterone downregulates the secretion of LIF in the decidua during early pregnancy.
小鼠子宫白血病抑制因子（LIF）的表达受母体激素调控，且不依赖胚胎的存在。本研究的目的是调查在人类中，妊娠早期LIF的分泌是否受母体控制，以及其产生是否与妊娠激素、孕酮和β-人绒毛膜促性腺激素（β-hCG）相关。为排除蜕膜与滋养层旁分泌相互作用的可能性，我们检测了宫外孕女性体内LIF的分泌。本研究设计为一项前瞻性、盲法、临床和免疫生化研究。对12名宫外孕手术女性进行了子宫内膜活检。同一天，检测母体血浆中孕酮和β-hCG的水平。测定了培养的蜕膜外植体上清液中的LIF浓度。在所有宫外孕女性中均发现蜕膜培养外植体产生LIF（中位数5015 pg，范围1389 - 19304 pg）。LIF产生量与妊娠孕周或循环β-hCG水平之间无相关性（p>0.05）。然而，当循环血浆中孕酮浓度低于5 ng/ml时，LIF的分泌量比孕酮水平高于5 ng/ml的女性高2.3倍（p<0.01）。因此，我们得出结论，人蜕膜可主动产生LIF，且这种细胞因子的产生不依赖于胎儿滋养层的存在。本研究首次证明，孕酮在妊娠早期可下调蜕膜中LIF的分泌。
