Endo T, Henmi H, Goto T, Kitajima Y, Kiya T, Nishikawa A, Manase K, Yamamoto H, Kudo R
Department of Obstetrics and Gynecology, School of Medicine, Sapporo Medical University, Japan.
Gynecol Endocrinol. 1998 Feb;12(1):29-34. doi: 10.3109/09513599809024967.
The human corpus luteum produces both estradiol and progesterone. It is well known that there are both autocrine and paracrine systems for the regulation of the corpus luteum and that estradiol regulates the progesterone production of the corpora lutea of some other species. To assess the direct effects of estrogen on human luteal function, we performed cell culture experiments. A low concentration of estradiol, almost equal to the amount of estradiol produced by human cultured luteal cells, directly stimulated progesterone production. 4-Cyclohexylaniline, an aromatase inhibitor, significantly reduced both progesterone production and estradiol production. Levels of estradiol higher than the levels that cultured human luteal cells themselves produced significantly reduced basal progesterone production and also significantly reduced human chorionic gonadotropin (hCG), forskolin and dibutyryl-cyclic AMP-stimulated progesterone production. According to these data, high doses of estradiol produced a luteolytic action which widely inhibited the steroidogenesis process. In conclusion, our results indicated that estradiol in part regulates progesterone production physiologically and blocks progesterone production in a pharmacological or pathological state in the human corpus luteum.
人类黄体可产生雌二醇和孕酮。众所周知，存在自分泌和旁分泌系统来调节黄体，并且雌二醇可调节其他一些物种黄体的孕酮生成。为了评估雌激素对人类黄体功能的直接影响，我们进行了细胞培养实验。低浓度的雌二醇，几乎等同于人类培养的黄体细胞产生的雌二醇量，直接刺激了孕酮的生成。芳香化酶抑制剂4-环己基苯胺显著降低了孕酮生成和雌二醇生成。高于人类培养的黄体细胞自身产生水平的雌二醇显著降低了基础孕酮生成，并且也显著降低了人绒毛膜促性腺激素（hCG）、福斯可林和二丁酰环磷腺苷刺激的孕酮生成。根据这些数据，高剂量的雌二醇产生了溶黄体作用，广泛抑制了类固醇生成过程。总之，我们的结果表明，雌二醇在生理上部分调节孕酮生成，并在人类黄体处于药理或病理状态时阻断孕酮生成。
