Ciusani Emilio, Frigerio Simona, Gelati Maurizio, Corsini Elena, Dufour Anna, Nespolo Angelo, La Mantia Loredana, Milanese Clara, Massa Giorgio, Salmaggi Andrea
Istituto Nazionale Neurologico `C. Besta', Via Celoria 11, 20133 Milan, Italy.
J Neuroimmunol. 1998 Feb;82(1):5-12. doi: 10.1016/S0165-5728(97)00177-X.
CSF and serum levels of soluble Fas were studied in MS patients, in patients with various neurological diseases and in healthy controls. We did not detect differences in serum sFas levels between MS patients and controls. In CSF, despite sFas levels being similar in all patients studied, a statistically significant correlation between sFas CSF/sFas serum ratio and BBB damage (expressed as albumin CSF/albumin serum ratio) was detected in non-MS neurological disease, but not in MS patients. The normalized ratio (sFas CSF/sFas serum)/(Alb CSF/Alb serum) was significantly increased in MS patients compared to patients with non-inflammatory neurological disease suggesting an intrathecal synthesis of soluble Fas in MS. The percentage of apoptotic mononuclear cells was higher in CSF as compared to peripheral blood; moreover a lower proportion of apoptotic cells was found in CSF of MS patients. The findings lend support to the involvement of sFas in MS pathogenesis and suggest that a lower apoptosis in CSF may be a feature of the disease.
在多发性硬化症（MS）患者、患有各种神经疾病的患者以及健康对照者中研究了脑脊液（CSF）和血清中可溶性Fas的水平。我们未检测到MS患者与对照者血清可溶性Fas水平存在差异。在脑脊液中，尽管所有研究患者的可溶性Fas水平相似，但在非MS神经疾病患者中检测到可溶性Fas脑脊液/可溶性Fas血清比值与血脑屏障（BBB）损伤（以脑脊液白蛋白/血清白蛋白比值表示）之间存在统计学显著相关性，而在MS患者中未检测到。与非炎性神经疾病患者相比，MS患者的标准化比值（可溶性Fas脑脊液/可溶性Fas血清）/（白蛋白脑脊液/白蛋白血清）显著升高，提示MS中可溶性Fas的鞘内合成。与外周血相比，脑脊液中凋亡单核细胞的百分比更高；此外，在MS患者的脑脊液中发现凋亡细胞的比例较低。这些发现支持可溶性Fas参与MS发病机制，并表明脑脊液中较低的细胞凋亡可能是该疾病的一个特征。
