Tipold A, Moore P, Jungi T W, Sager H, Vandevelde M
Institute of Animal Neurology, University of Bern, Bern, Switzerland; Institute of Veterinary Virology, University of Bern, Bern, Switzerland.
VM Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA.
J Neuroimmunol. 1998 Feb;82(1):90-95. doi: 10.1016/S0165-5728(97)00192-6.
In order to evaluate changes in lymphocyte subpopulations of cerebrospinal fluid (CSF) cells in neurological diseases, normal control data have to be established. In this study we evaluated CSF samples from 65 dogs of both sexes and various breeds with an age range between 5 months and 6 years, and 20 one-year-old healthy inbred Beagles. For comparison, blood samples from 10 healthy dogs were examined. 14 different antibodies against leukocyte surface markers were used. The subpopulations were evaluated using flow cytometry (FACS) and immunocytochemistry. It could be shown that lymphocyte populations in CSF differ from peripheral blood in a few subsets. A relatively high degree of individual variation was found, not only in dogs of different breeds and ages, but also in the inbred Beagle population. These large individual variations suggest that repeated paired CSF-blood samples during the course of neurological disease should be examined within the same individual to obtain meaningful results. CD3+ and CD4+ T-cells were significantly lower in normal CSF. Of great interest is the fact, that T-cells, characterized by double staining CD3/CD45RA are present in variable numbers in normal CSF. In other species they are known to be naive or resting T-cells. CD4/CD45RA positive cells seem to be an important subpopulation of these CD45RA positive T-cells. Furthermore, by far more CD11b positive lymphocytes were observed in the CSF than in the peripheral blood and these are not large granular lymphocytes. The present study shows that systematic FACS analysis of CSF is feasible in larger animals such as dogs.
为了评估神经疾病中脑脊液（CSF）细胞淋巴细胞亚群的变化，必须建立正常对照数据。在本研究中，我们评估了65只年龄在5个月至6岁之间、雌雄不限、品种各异的犬以及20只1岁健康近交系比格犬的脑脊液样本。作为对照，检测了10只健康犬的血液样本。使用了14种针对白细胞表面标志物的不同抗体。通过流式细胞术（FACS）和免疫细胞化学评估亚群。结果表明，脑脊液中的淋巴细胞群在一些亚群上与外周血不同。不仅在不同品种和年龄的犬中，而且在近交系比格犬群体中都发现了相对较高程度的个体差异。这些较大的个体差异表明，在神经疾病过程中，应在同一个体内检查重复的配对脑脊液 - 血液样本，以获得有意义的结果。正常脑脊液中CD3 +和CD4 + T细胞显著降低。有趣的是，以CD3/CD45RA双重染色为特征的T细胞在正常脑脊液中数量不等。在其他物种中，它们被认为是幼稚或静止的T细胞。CD4/CD45RA阳性细胞似乎是这些CD45RA阳性T细胞的一个重要亚群。此外，脑脊液中观察到的CD11b阳性淋巴细胞远比外周血中的多，且这些不是大颗粒淋巴细胞。本研究表明，对脑脊液进行系统的FACS分析在犬等大型动物中是可行的。
