Svenningsson A, Andersen O, Edsbagge M, Stemme S
Department of Clinical Neuroscience, University of Gothenburg, Sahlgren's University Hospital, Sweden.
J Neuroimmunol. 1995 Dec;63(1):39-46. doi: 10.1016/0165-5728(95)00126-3.
The distribution of lymphocyte subpopulations in cerebrospinal fluid (CSF) and their phenotypic characteristics were extensively investigated in a group of 18 healthy individuals using two- and three-color flow cytometry. Generally, CD3+ T lymphocytes constituted the vast majority of CSF lymphocytes while the number of B lymphocytes and NK cells were low. Most T lymphocytes exhibited the phenotype of memory/primed cells in both the CD4+ and CD8+ subpopulations. Two markers for recent activation, HLA-DR and interleukin-2 receptor (CD25) were not upregulated when compared with peripheral blood (PB) in the majority of CSF T lymphocytes. However, a fraction of T lymphocytes co-expressing the NK cells markers CD56 and/or CD16 showed a pronounced upregulation of HLA-DR in CSF as compared with PB. This study documents that the cellular composition of the normal CSF differs profoundly from PB regarding all major lymphocyte subpopulations. This has to be taken into account in studies addressing questions regarding cellular immune reactions in the central nervous system under pathological conditions.
利用双色和三色流式细胞术，对18名健康个体的脑脊液（CSF）中淋巴细胞亚群的分布及其表型特征进行了广泛研究。一般来说，CD3 + T淋巴细胞占CSF淋巴细胞的绝大多数，而B淋巴细胞和NK细胞数量较少。大多数T淋巴细胞在CD4 +和CD8 +亚群中均表现出记忆/致敏细胞的表型。与外周血（PB）相比，大多数CSF T淋巴细胞中近期活化的两个标志物HLA - DR和白细胞介素 - 2受体（CD25）未上调。然而，与PB相比，一小部分共表达NK细胞标志物CD56和/或CD16的T淋巴细胞在CSF中HLA - DR明显上调。本研究证明，正常CSF的细胞组成在所有主要淋巴细胞亚群方面与PB有很大差异。在研究病理条件下中枢神经系统细胞免疫反应的问题时，必须考虑到这一点。
