Luxembourg A T, Borrow P, Teyton L, Brunmark A B, Peterson P A, Jackson M R
R.W. Johnson Pharmaceutical Research Institute, San Diego, CA 92121, USA.
Nat Biotechnol. 1998 Mar;16(3):281-5. doi: 10.1038/nbt0398-281.
Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility complex class I molecules loaded with specific peptide were used as a substrate for T-cell capture. Low-frequency T cells, as well as T cells with TCR of low affinity for the antigen were captured on the beads. Following isolation and expansion, recovered cells specifically killed target cells in vitro, and displayed antiviral effect in vivo.
由于细胞频率低以及T细胞受体（TCR）与抗原之间的亲和力低，分离抗原特异性T淋巴细胞受到阻碍。我们描述了从混合T细胞群体中分离和纯化抗原特异性CD8 + T淋巴细胞的方法。涂有负载特定肽的主要组织相容性复合体I类分子的磁珠用作T细胞捕获的底物。低频T细胞以及对抗原亲和力低的TCR的T细胞被捕获在磁珠上。分离和扩增后，回收的细胞在体外特异性杀死靶细胞，并在体内显示出抗病毒作用。
