Zittoun R, Pochat L, Mathieu M, Pochat R
Pathol Biol (Paris). 1981 May;29(5):317-20.
The reliability of DNA cell content measurement by flow cytophotometry was assessed after simultaneous staining of DNA and RNA by acridine-orange. The methods of staining allowing the lowest CV were determined. The specificity of this staining for DNA appeared satisfactory. When the distribution in the various phases of the cell cycle was computerized, a good correlation was found for the S phase with the 3H-thymidine labeling index. The interest of this method for prognosis and monitoring of acute leukemia is underlined.
通过吖啶橙同时对DNA和RNA进行染色后，评估了流式细胞光度法测量DNA细胞含量的可靠性。确定了具有最低变异系数的染色方法。这种对DNA染色的特异性似乎令人满意。当对细胞周期各阶段的分布进行计算机分析时，发现S期与3H-胸腺嘧啶标记指数具有良好的相关性。强调了该方法对急性白血病预后和监测的重要性。
