Gutzwiller A
Eidgenössische Forschungsanstalt für Nutztiere, Posieux.
Schweiz Arch Tierheilkd. 1998;140(3):120-4.
In order to evaluate a dipstick test for the semi-quantitative determination of beta-hydroxybutyrate (BHB) in milk, we determined the BHB content in milk samples of healthy and ketotic high yielding dairy cows with the dipsticks. BHB was analysed on an analyser in 39 of these milk samples as well as in 88 blood samples which were drawn when milk samples were obtained. There was a strong correlation between milk BHB values which were determined with the two methods (r = 0.92; BHB quantitative = -9.9 + 1.25 x BHB dipstick value). Although BHB concentration in plasma is much higher than in milk, the correlation between plasma BHB, determined on an analyser, and the dipstick test results in the corresponding milk samples was also quite strong (r = 0.84; plasma BHB in mmol/l = 0.23 + 0.009 x milk BHB in mumol/l, determined with dipsticks). The dipstick test is suitable for the diagnosis of clinical ketosis and for monitoring the energy status of high yielding dairy cows.
为评估用于半定量测定牛奶中β-羟基丁酸（BHB）的试纸条检测方法，我们使用该试纸条测定了健康高产奶牛和酮病高产奶牛牛奶样本中的BHB含量。在其中39份牛奶样本以及采集牛奶样本时抽取的88份血液样本中，用分析仪对BHB进行了分析。两种方法测定的牛奶BHB值之间存在很强的相关性（r = 0.92；BHB定量值=-9.9 + 1.25×BHB试纸条检测值）。尽管血浆中的BHB浓度远高于牛奶中的浓度，但用分析仪测定的血浆BHB与相应牛奶样本试纸条检测结果之间的相关性也很强（r = 0.84；血浆BHB（mmol/l）= 0.23 + 0.009×用试纸条测定的牛奶BHB（μmol/l））。该试纸条检测方法适用于临床酮病的诊断以及监测高产奶牛的能量状态。
