James V J, McConnell J F, Amemiya Y
Department of Biophysics, University of New South Wales, Sydney, Australia.
Biochim Biophys Acta. 1998 Feb 2;1379(2):282-8. doi: 10.1016/s0304-4165(97)00108-6.
Low-angle synchrotron X-ray diffraction studies of human fetal extensor tendon and skin collagen, centred in time about the period of first major movement by the fetus, indicate that alignment of the tendon collagen fibrils occurs about this time. At this stage there appears to be no detectable structural difference between tendon and dermis. By three weeks post-partum, marked differences between these tissues can be detected. The distribution of the intermediate filament diameters for all fetal tendons investigated was unimodal (mean 41.2 +/- 0.4 nm) in contrast with that for post-partum tendon which is multimodal. Equatorial periodicities of 353 +/- 3 nm and 32.1 +/- 0.1 nm, consistent with the presence of type IV collagen, were obtained from all fetal samples examined. Neither of these periodicities were observed in post-partum normal tendon and only the larger were observed in post-partum normal skin. The consistency of the results suggest that low-angle X-ray diffraction could be used for the identification of fetal-like tissues found in pathological tissues.
对人类胎儿伸肌腱和皮肤胶原蛋白进行的低角度同步加速器X射线衍射研究，以胎儿首次主要活动时期为时间中心，结果表明肌腱胶原纤维的排列大约在这个时候发生。在这个阶段，肌腱和真皮之间似乎没有可检测到的结构差异。产后三周时，可以检测到这些组织之间的明显差异。与产后肌腱的多峰分布不同，所有研究的胎儿肌腱中间丝直径的分布是单峰的（平均41.2 +/- 0.4纳米）。从所有检查的胎儿样本中获得了与IV型胶原蛋白存在一致的353 +/- 3纳米和32.1 +/- 0.1纳米的赤道周期。在产后正常肌腱中未观察到这些周期中的任何一个，在产后正常皮肤中仅观察到较大的周期。结果的一致性表明，低角度X射线衍射可用于识别病理组织中发现的类似胎儿的组织。
