Azuma N, Izumi T, Tajima S, Nishikawa T, Ohshima A
Department of Ophthalmology, National Children's Hospital, Keio University School of Medicine, Tokyo.
J Invest Dermatol. 1994 Jun;102(6):958-62. doi: 10.1111/1523-1747.ep12384129.
Using in situ hybridization techniques and RNase protection assays, type II collagen mRNA was transiently detected in the epidermis of chick embryonic skins during days 9-15 after fertilization, with a maximum expression at day 11. Immunohistochemical studies demonstrated that deposition of type II collagen was also transiently localized at the subepidermal region during days 10-15. Type II collagen gene and gene product concomitantly started to decline preferentially at the region where feather buds were being formed on day 12, and thereafter diminished at the region between feather buds. Using immunohistochemical methods, type II collagen was also detected in human fetal scalp skin at 17-23 fetal weeks at the subepidermal region, excluding the region beneath the hair follicles. These results indicate that the lack of type II collagen expression is related to the development of feather and hair at a certain stage of chick embryonic and human fetal skin development.
运用原位杂交技术和核糖核酸酶保护分析，在受精后第9至15天的鸡胚皮肤表皮中短暂检测到II型胶原蛋白信使核糖核酸，在第11天表达量最高。免疫组织化学研究表明，在第10至15天期间，II型胶原蛋白的沉积也短暂地定位于表皮下区域。在第12天，II型胶原蛋白基因及其基因产物在羽芽形成的区域优先开始同时下降，此后在羽芽之间的区域减少。采用免疫组织化学方法，在17至23周胎儿的人胎儿头皮皮肤的表皮下区域（不包括毛囊下方区域）也检测到了II型胶原蛋白。这些结果表明，II型胶原蛋白表达的缺失与鸡胚和人胎儿皮肤发育特定阶段羽毛和毛发的发育有关。
