与阳离子脂质体结合的DNA复合物的结构——圆柱形双层还是扁平双层？

The structure of DNA complexes with cationic liposomes-cylindrical or flat bilayers?

作者信息

Dan N

机构信息

Dept. of Chemical Engineering, University of Delaware, Newark 19716, USA.

出版信息

Biochim Biophys Acta. 1998 Feb 2;1369(1):34-8. doi: 10.1016/s0005-2736(97)00171-5.

DOI:10.1016/s0005-2736(97)00171-5

PMID:9528671

Abstract

DNA complexes with cationic lipids promise to be versatile and effective synthetic transfection agents. Recent experiments identified both flat lamellar structures, where DNA strands are sandwiched between lipid bilayers, and cylindrical ones where the DNA is coated by a curved bilayer. Using a simple model we compare the stability of the two structures, and find that flat-bilayer aggregates are always more stable than the cylindrical ones. The different experimental observations are explained within the framework of the model predictions.

摘要

与阳离子脂质形成的DNA复合物有望成为通用且有效的合成转染剂。最近的实验发现了两种结构，一种是扁平的层状结构，其中DNA链夹在脂质双层之间；另一种是圆柱形结构，其中DNA被弯曲的双层包裹。我们使用一个简单的模型比较了这两种结构的稳定性，发现扁平双层聚集体总是比圆柱形聚集体更稳定。在模型预测的框架内解释了不同的实验观察结果。