Omigbodun A, Coukos G, Ziolkiewicz P, Wang C L, Coutifaris C
Department of Obstetrics and Gynecology, and the Center for Research in Reproduction and Women's Health, University of Pennsylvania, Philadelphia 19104, USA.
Endocrinology. 1998 Apr;139(4):2190-3. doi: 10.1210/endo.139.4.6031.
Macrophage-colony stimulating factor (M-CSF, CSF-1) is secreted by human trophoblasts as well as endometrial cells, while its receptor c-fms is abundantly expressed by the extravillous trophoblasts anchoring the placenta to the uterus, suggesting a role for M-CSF at the maternal-fetal interface. We investigated the effect of M-CSF on the expression of fibronectin and its receptor, the alpha5 integrin, in human trophoblasts. Exposure of trophoblasts to M-CSF produced a two to three-fold increase in fibronectin and alpha5 mRNA abundance at both 24 and 72 hours of culture. A dose-dependent increase in cellular fibronectin secretion into the culture medium was detected at both time points. Immunocytochemistry showed co-localization of cellular fibronectin and alpha5 in the cells, suggesting that attachment of trophoblasts to fibronectin is mediated in part by the alpha5beta1 integrin. We conclude that M-CSF increases fibronectin expression and secretion by the human trophoblasts, and up-regulates its specific receptor, the alpha5 integrin. We hypothesize that M-CSF may partake in the autocrine/paracrine mechanisms regulating trophoblast invasion during implantation.
巨噬细胞集落刺激因子（M-CSF，CSF-1）由人滋养层细胞以及子宫内膜细胞分泌，而其受体c-fms在将胎盘锚定到子宫的绒毛外滋养层细胞中大量表达，这表明M-CSF在母胎界面发挥作用。我们研究了M-CSF对人滋养层细胞中纤连蛋白及其受体α5整合素表达的影响。在培养24小时和72小时时，将滋养层细胞暴露于M-CSF会使纤连蛋白和α5 mRNA丰度增加两到三倍。在这两个时间点均检测到培养基中细胞纤连蛋白分泌呈剂量依赖性增加。免疫细胞化学显示细胞纤连蛋白和α5在细胞中共定位，这表明滋养层细胞与纤连蛋白的附着部分由α5β1整合素介导。我们得出结论，M-CSF可增加人滋养层细胞中纤连蛋白的表达和分泌，并上调其特异性受体α5整合素。我们推测M-CSF可能参与了植入过程中调节滋养层细胞侵袭的自分泌/旁分泌机制。
