铜绿假单胞菌对人肠道Caco-2上皮细胞单层的黏附和穿透
Adherence to and penetration of human intestinal Caco-2 epithelial cell monolayers by Pseudomonas aeruginosa.
作者信息
Hirakata Y, Izumikawa K, Yamaguchi T, Igimi S, Furuya N, Maesaki S, Tomono K, Yamada Y, Kohno S, Yamaguchi K, Kamihira S
机构信息
Department of Laboratory Medicine, Nagasaki University School of Medicine, Sakamoto, Japan.
出版信息
Infect Immun. 1998 Apr;66(4):1748-51. doi: 10.1128/IAI.66.4.1748-1751.1998.
Abstract
Clinical isolates of Pseudomonas aeruginosa from blood adhered to and penetrated intestinal Caco-2 cell monolayers to a greater degree than did isolates from sputum, with a concomitant drastic decrease in transepithelial electrical resistance. PAO-PR1, an avirulent exotoxin A mutant of PAO1, did not cause a decrease in the resistance. The Caco-2 monolayer system may be useful for the evaluation of certain P. aeruginosa virulence factor activities.
摘要
血液中分离出的铜绿假单胞菌临床菌株比痰液中分离出的菌株更易黏附并穿透肠道Caco-2细胞单层，同时跨上皮电阻急剧下降。PAO-PR1是PAO1的无毒外毒素A突变体，不会导致电阻下降。Caco-2单层系统可能有助于评估某些铜绿假单胞菌毒力因子的活性。
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