Skalka B, Literák I, Michalík I, Skrivánek M
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Zentralbl Veterinarmed B. 1998 Feb;45(1):31-5. doi: 10.1111/j.1439-0450.1998.tb00763.x.
Prevalence of caseous lymphadenitis was studied in goats from six herds in the Czech Republic. Corynebacterium pseudotuberculosis exhibiting typical properties was isolated from clinically affected animals only. Antibodies to C. pseudotuberculosis were identified by means of agar immunodiffusion and the neutralization test using a toxin--phospholipase D (PLD)--as antigen. No clinical manifestations of caseous lymphadenitis or antibodies to C. pseudotuberculosis were found in four herds. In one herd without any history of clinical caseous lymphadenitis, serological positivity was reported in two out of 148 examinations. In a herd with clinical manifestations of caseous lymphadenitis, antibodies were ascertained to a various degree in subsequent samplings (100%, 87% and 64%, respectively). The importance of serologic examination of caseous lymphadenitis in goat herds is discussed.
对捷克共和国六个牧群中的山羊进行了干酪性淋巴结炎患病率的研究。仅从临床患病动物中分离出具有典型特性的伪结核棒状杆菌。通过琼脂免疫扩散和使用毒素——磷脂酶D(PLD)作为抗原的中和试验来鉴定抗伪结核棒状杆菌抗体。在四个牧群中未发现干酪性淋巴结炎的临床表现或抗伪结核棒状杆菌抗体。在一个没有任何临床干酪性淋巴结炎病史的牧群中,148次检测中有两次报告血清学呈阳性。在一个有干酪性淋巴结炎临床表现的牧群中,在随后的采样中分别在不同程度上检测到抗体(分别为100%、87%和64%)。讨论了山羊群中干酪性淋巴结炎血清学检查的重要性。