Sting R, Steng G, Spengler D
Zentralbl Veterinarmed B. 1998 May;45(4):209-16. doi: 10.1111/j.1439-0450.1998.tb00785.x.
Corynebacterium pseudotuberculosis was isolated from a goat suffering from caseous lymphadenitis and used for preparation of cell wall antigens and exotoxin for detection of specific antibodies in enzyme-linked immunosorbent assay (ELISA). In immunoblotting sodium dodecyt sulphate (SDS) extracted cell wall antigens revealed molecular weights ranging from 20 to 120 kDa. The raw exotoxin showed molecular weights of 30 and 55 kDa and an inhibition of the haemolysis of a Staphylococcus aureus strain. For validation of the ELISA 109 goats of known clinical status were examined reaching a sensitivity of 96% and a specificity of 76% for this test. No serological reactions showed in 191 goats originating from 11 flocks which never had suffered from caseous lymphadenitis. Using this ELISA test 24 goats originating from flocks suffering from caseous lymphadenitis were examined serologically before and after vaccination with a bacterin. Before vaccination one of the five goats with clinical signs showed no positive reaction in ELISA. After vaccination all 24 animals showed positive reactions. Of a total of 1868 goats sampled in Baden-Wuerttemberg 41 (2.2%) in 22 (10%) flocks showed positive reactions in ELISA.
从一只患有干酪性淋巴结炎的山羊中分离出伪结核棒状杆菌，并用于制备细胞壁抗原和外毒素，以在酶联免疫吸附测定（ELISA）中检测特异性抗体。在免疫印迹中，十二烷基硫酸钠（SDS）提取的细胞壁抗原显示分子量范围为20至120 kDa。粗制外毒素显示分子量为30和55 kDa，并对金黄色葡萄球菌菌株的溶血有抑制作用。为验证ELISA，对109只已知临床状态的山羊进行了检测，该检测的敏感性为96%，特异性为76%。来自11个从未患过干酪性淋巴结炎的羊群的191只山羊未出现血清学反应。使用该ELISA检测对来自患有干酪性淋巴结炎的羊群的24只山羊在接种菌苗前后进行了血清学检测。接种前，五只出现临床症状的山羊中有一只在ELISA中未显示阳性反应。接种后，所有24只动物均显示阳性反应。在巴登-符腾堡州采样的总共1868只山羊中，22个（10%）羊群中的41只（2.2%）在ELISA中显示阳性反应。
