Symon Z, Fuchs S, Agmon Y, Weiss O, Nephesh I, Moshe R, Brezis M, Flyvbjerg A, Raz I
Department of Medicine, Hadassah University Hospital, Jerusalem, Israel.
Am J Physiol. 1998 Mar;274(3):F490-7. doi: 10.1152/ajprenal.1998.274.3.F490.
The response of insulin-like growth factor (IGF) I in acute renal failure was evaluated in a model of radiocontrast nephropathy associated with selective necrosis of medullary thick ascending limbs. In brief, rats were administered radiocontrast medium or vehicle injections for controls after combined inhibition of prostanoids and nitric oxide. Twenty-four hours after the insult, tissue mRNAs for IGF-I, the IGF-I receptor, and IGF-binding proteins (IGFBP) 1 and 3 were assayed in cortex, medulla, and liver by solution hybridization-RNase protection assay, and IGFBPs were measured in serum and tissue by Western ligand blotting. Cortical IGF-1 increased, whereas medullary IGF-I mRNA decreased. Renal IGFBPs decreased, whereas IGFBP-1 mRNA increased. The IGF system in the liver was unchanged. We conclude that general changes in renal IGFBPs in this experimental model of acute renal failure might increase the level of cortical IGF-I in a way that could modulate medullary recovery.
在与髓质厚升支选择性坏死相关的放射性造影剂肾病模型中，评估了胰岛素样生长因子（IGF）I在急性肾衰竭中的反应。简而言之，在联合抑制前列腺素和一氧化氮后，给大鼠注射放射性造影剂或作为对照的赋形剂。损伤后24小时，通过溶液杂交-核糖核酸酶保护试验在皮质、髓质和肝脏中检测IGF-I、IGF-I受体以及IGF结合蛋白（IGFBP）1和3的组织mRNA，并通过Western配体印迹法在血清和组织中测量IGFBP。皮质IGF-1增加，而髓质IGF-I mRNA减少。肾IGFBP减少，而IGFBP-1 mRNA增加。肝脏中的IGF系统未发生变化。我们得出结论，在这个急性肾衰竭实验模型中，肾IGFBP的总体变化可能会以一种能够调节髓质恢复的方式增加皮质IGF-I的水平。
