BALB/c成纤维细胞悬液中一种外甘露糖基转移酶催化甘露糖脂的形成。

Formation of mannosyl-lipids by an ectomannosyltransferase in suspensions of BALB/c fibroblasts.

作者信息

Patt L M, Grimes W J

出版信息

Biochim Biophys Acta. 1976 Aug 24;444(1):97-107. doi: 10.1016/0304-4165(76)90227-0.

DOI:10.1016/0304-4165(76)90227-0

Abstract

A mannosyltransferase has been detected in suspensions of BALB/c fibroblasts incubated with GDP-[14C] mannose. The experimental evidence indicates the cell surface as the most likely site for the enzyme. The transferase synthesizes both glycolipids and glycoproteins. The lipid compounds have properties suggestive of lipid-linked mono- and oligosaccharides which can function as intermediates in glycoprotein synthesis. The formation of these compounds by a cell surface enzyme suggests that lipid-linked intermediates may play an important role in the glycosylation of membrane components.

摘要

在用GDP-[14C]甘露糖孵育的BALB/c成纤维细胞悬液中检测到了一种甘露糖基转移酶。实验证据表明，细胞表面是该酶最可能的作用位点。该转移酶可合成糖脂和糖蛋白。这些脂质化合物具有脂质连接的单糖和寡糖的特性，它们可作为糖蛋白合成的中间体发挥作用。细胞表面酶形成这些化合物表明，脂质连接的中间体可能在膜成分的糖基化过程中发挥重要作用。

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