大鼠1号和4号染色体上体重数量性状基因座的定位

Mapping of quantitative trait loci for body weight on chromosomes 1 and 4 in the rat.

作者信息

Kovács P, Klöting I

机构信息

Department of Laboratory Animal Science, University of Greifswald, Karlsburg, Germany.

出版信息

Biochem Mol Biol Int. 1998 Feb;44(2):399-405. doi: 10.1080/15216549800201412.

DOI:10.1080/15216549800201412

PMID:9530522

Abstract

Quantitative trait loci (QTLs) affecting body weight were investigated in the backcross population derived from diabetic BB/OK and spontaneously hypertensive rat (SHR). The F1 hybrids were backcrossed onto BB/OK rats, and QTL analysis was performed with the resulting backcross population on chromosomes 1, 3, 4, 10, 13 and 18. According to the stringent threshold for a lod score of 3.0, markers on chromosomes 1 and 4 were found to be linked with body weight. The QTL with a peak lod score (3.3) on chromosome 1 for a male population was located within the region flanked by loci Igf2 and D1Mgh12. On chromosome 4, linkage between the body weight and the region around the Npy locus was observed (lod score 3.1). The existence of the QTL on chromosome 4 affecting body weight was confirmed by congenic BB.LL rats, carrying chromosomal region of SHR (D4Mit6-Npy-Spr) on the genetic background of the BB/OK rat.

摘要

在源自糖尿病BB/OK大鼠和自发性高血压大鼠（SHR）的回交群体中，对影响体重的数量性状基因座（QTL）进行了研究。将F1杂种与BB/OK大鼠回交，并对所得回交群体在1、3、4、10、13和18号染色体上进行QTL分析。根据对数优势得分3.0的严格阈值，发现1号和4号染色体上的标记与体重相关。雄性群体在1号染色体上具有峰值对数优势得分（3.3）的QTL位于由Igf2和D1Mgh12基因座侧翼的区域内。在4号染色体上，观察到体重与Npy基因座周围区域之间的连锁关系（对数优势得分3.1）。携带SHR染色体区域（D4Mit6-Npy-Spr）的同基因BB.LL大鼠在BB/OK大鼠的遗传背景下，证实了4号染色体上影响体重的QTL的存在。