Shoyama Y, Kawachi F, Tanaka H, Nakai R, Shibata T, Nishi K
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Forensic Sci Int. 1998 Feb 16;91(3):207-17. doi: 10.1016/s0379-0738(97)00196-5.
Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
从一个F1杂种及其亲本植物苞叶罂粟（Papaver bracteatum Lindle）和伪东方罂粟（P. pseudo-orientale Medw.）的叶片中提取了总DNA。使用10种任意的10聚体寡核苷酸对随机扩增多态性DNA（RAPD）进行分析，结果表明，与F1杂种和单个亲本之间的遗传距离相比，F1杂种被确认为是两个亲本植物的遗传中间类型。此外，F1杂种、苞叶罂粟和伪东方罂粟之间条带模式的比较清楚地表明，两个亲本的部分条带被导入到了F1杂种中。使用抗蒂巴因单克隆抗体通过高效液相色谱法（HPLC）和酶联免疫吸附测定法（ELISA）测定了蒂巴因的含量。
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