用于计算复杂生化混合物平衡组成的定点方法。
Fixed-point methods for computing the equilibrium composition of complex biochemical mixtures.
作者信息
Kuzmic P
机构信息
BioKin Consulting, P.O. Box 8336, Madison, WI 53708, USA.
出版信息
Biochem J. 1998 Apr 15;331 ( Pt 2)(Pt 2):571-5. doi: 10.1042/bj3310571.
Abstract
The fixed-point algebraic method [Storer and Cornish-Bowden (1976) Biochem. J. 159, 1-5] for computing the concentrations at equilibrium of complex biochemical mixtures fails for many binding stoichiometries, especially those that include molecular self-association. A typical example is the monomer-dimer-tetramer equilibrium. This paper reports two main results. First, the above algorithm is analysed theoretically to predict for which binding stoichiometries it succeeds and for which it will fail. Secondly, an alternative algorithm is described for self-associating biochemical systems. Illustrative examples are based on the dimeric proteinase from HIV.
摘要
用于计算复杂生化混合物平衡浓度的定点代数法[斯托勒和康沃尔-鲍登（1976年），《生物化学杂志》159卷，第1 - 5页]在许多结合化学计量比情况下都会失效，尤其是那些涉及分子自缔合的情况。一个典型例子是单体 - 二聚体 - 四聚体平衡。本文报告了两个主要结果。首先，从理论上分析上述算法，以预测它在哪些结合化学计量比情况下会成功，哪些情况下会失败。其次，描述了一种用于自缔合生化系统的替代算法。示例基于来自HIV的二聚体蛋白酶。
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