染色体分离过程中大肠杆菌类核中复制起点和终点的极性定位。
Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning.
作者信息
Niki H, Hiraga S
机构信息
Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862-0976, Japan.
出版信息
Genes Dev. 1998 Apr 1;12(7):1036-45. doi: 10.1101/gad.12.7.1036.
Abstract
We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC migrates rapidly to the opposite nucleoid border. These oriC copies are retained at both nucleoid borders, remaining at a constant distance from each cell pole. The terminus segment migrates from the nucleoid border to midcell and is retained there until the terminus is duplicated. The origin, terminus and other DNA regions show three migration patterns during active partitioning of daughter chromosomes.
摘要
我们通过荧光原位杂交（FISH）展示了细胞分裂周期中大肠杆菌复制起点（oriC）和染色体末端的细胞内定位。在新生细胞中，oriC定位于旧极近端的类核边界，而末端定位于新极近端的类核边界。复制后的oriC的一个拷贝迅速迁移到相对的类核边界。这些oriC拷贝保留在两个类核边界处，与每个细胞极保持恒定距离。末端片段从类核边界迁移到细胞中部并保留在那里，直到末端被复制。在子染色体的活跃分配过程中，起点、末端和其他DNA区域呈现出三种迁移模式。
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本文引用的文献
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