细菌染色体的组织与分离。
Bacterial chromosome organization and segregation.
机构信息
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, California 94305, USA.
出版信息
Cold Spring Harb Perspect Biol. 2010 Feb;2(2):a000349. doi: 10.1101/cshperspect.a000349.
Abstract
Bacterial chromosomes are generally approximately 1000 times longer than the cells in which they reside, and concurrent replication, segregation, and transcription/translation of this crowded mass of DNA poses a challenging organizational problem. Recent advances in cell-imaging technology with subdiffraction resolution have revealed that the bacterial nucleoid is reliably oriented and highly organized within the cell. Such organization is transmitted from one generation to the next by progressive segregation of daughter chromosomes and anchoring of DNA to the cell envelope. Active segregation by a mitotic machinery appears to be common; however, the mode of chromosome segregation varies significantly from species to species.
摘要
细菌染色体通常比其所在的细胞长约 1000 倍，而在如此拥挤的 DNA 大量存在的情况下，同时进行复制、分离和转录/翻译，这对组织提出了具有挑战性的问题。具有亚衍射分辨率的细胞成像技术的最新进展揭示了细菌类核在细胞内可靠地定向和高度组织。这种组织通过子染色体的渐进分离和 DNA 与细胞包膜的锚定，从一代传递到下一代。有丝分裂机制的主动分离似乎很常见；然而，染色体分离的模式在不同物种之间差异显著。
相似文献
1
Bacterial chromosome organization and segregation.细菌染色体的组织与分离。
Cold Spring Harb Perspect Biol. 2010 Feb;2(2):a000349. doi: 10.1101/cshperspect.a000349.
2
FtsK and SpoIIIE, coordinators of chromosome segregation and envelope remodeling in bacteria.FtsK和SpoIIIE，细菌中染色体分离和包膜重塑的协调因子。
Trends Microbiol. 2022 May;30(5):480-494. doi: 10.1016/j.tim.2021.10.002. Epub 2021 Oct 30.
3
Insensitivity of chromosome I and the cell cycle to blockage of replication and segregation of Vibrio cholerae chromosome II.霍乱弧菌染色体 II 的复制和分离阻断对染色体 I 不敏感性和细胞周期的影响。
mBio. 2012 May 8;3(3). doi: 10.1128/mBio.00067-12. Print 2012.
4
Bacterial partition complexes segregate within the volume of the nucleoid.细菌分隔复合物在拟核的体积内进行分隔。
Nat Commun. 2016 Jul 5;7:12107. doi: 10.1038/ncomms12107.
5
Resolving chromosome segregation in bacteria.解决细菌中的染色体分离问题。
J Mol Microbiol Biotechnol. 2006;11(3-5):126-39. doi: 10.1159/000094049.
6
Bacterial chromosome segregation.细菌染色体分离。
Front Biosci (Landmark Ed). 2012 Jan 1;17(3):1020-34. doi: 10.2741/3971.
7
Changes in nucleoid morphology and origin localization upon inhibition or alteration of the actin homolog, MreB, of Vibrio cholerae.霍乱弧菌肌动蛋白同源物MreB受到抑制或改变时，类核形态和复制起点定位的变化。
J Bacteriol. 2007 Oct;189(20):7450-63. doi: 10.1128/JB.00362-07. Epub 2007 Aug 17.
8
The role of co-transcriptional translation and protein translocation (transertion) in bacterial chromosome segregation.共转录翻译和蛋白质转运（转译转运）在细菌染色体分离中的作用。
Mol Microbiol. 2002 Jul;45(1):17-29. doi: 10.1046/j.1365-2958.2002.02993.x.
9
Replication initiator DnaA binds at the Caulobacter centromere and enables chromosome segregation.复制起始因子DnaA结合在柄杆菌着丝粒上并促进染色体分离。
Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):16100-5. doi: 10.1073/pnas.1418989111. Epub 2014 Oct 27.
10
Segregation of the replication terminus of the two Vibrio cholerae chromosomes.霍乱弧菌两条染色体复制终点的分离。
J Bacteriol. 2006 Feb;188(3):1060-70. doi: 10.1128/JB.188.3.1060-1070.2006.
引用本文的文献
1
Hypothesis that ancestral eukaryotes sexually proliferated without kinetochores or mitosis.关于原始真核生物在没有动粒或有丝分裂的情况下进行有性繁殖的假说。
J Cell Sci. 2025 Jun 1;138(11). doi: 10.1242/jcs.263843. Epub 2025 Jun 10.
2
A microfluidic platform for extraction and analysis of bacterial genomic DNA.一种用于提取和分析细菌基因组DNA的微流控平台。
Lab Chip. 2025 Mar 25;25(7):1767-1775. doi: 10.1039/d4lc00839a.
3
Nanotubes Formation in .在……中形成的纳米管。
Cells. 2022 Oct 26;11(21):3374. doi: 10.3390/cells11213374.
4
Proteolysis dependent cell cycle regulation in Caulobacter crescentus.新月柄杆菌中依赖蛋白酶解的细胞周期调控
Cell Div. 2022 Apr 1;17(1):3. doi: 10.1186/s13008-022-00078-z.
5
Whole-Genome Analysis Reveals That the Nucleoid Protein IHF Predominantly Binds to the Replication Origin Specifically at the Time of Initiation.全基因组分析表明，类核蛋白IHF主要在起始时特异性结合于复制起点。
Front Microbiol. 2021 Aug 12;12:697712. doi: 10.3389/fmicb.2021.697712. eCollection 2021.
6
Nonrandom segregation of sister chromosomes by MukBEF.MukBEF 导致姐妹染色体的非随机分离。
Proc Natl Acad Sci U S A. 2021 Aug 17;118(33). doi: 10.1073/pnas.2022078118.
7
A Hi-C data-integrated model elucidates E. coli chromosome's multiscale organization at various replication stages.高分辨率染色体构象捕获数据整合模型阐释了大肠杆菌染色体在不同复制阶段的多种尺度的结构。
Nucleic Acids Res. 2021 Apr 6;49(6):3077-3091. doi: 10.1093/nar/gkab094.
8
Replication and partitioning of the apicoplast genome of Toxoplasma gondii is linked to the cell cycle and requires DNA polymerase and gyrase.刚地弓形虫顶复体基因组的复制和分配与细胞周期有关，需要 DNA 聚合酶和拓扑异构酶。
Int J Parasitol. 2021 May;51(6):493-504. doi: 10.1016/j.ijpara.2020.11.004. Epub 2021 Feb 11.
9
Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis.玫红菌素的抗菌机制涉及猪链球菌中类核分离检查点的破坏。
AMB Express. 2020 Jun 8;10(1):110. doi: 10.1186/s13568-020-01047-x.
10
Deacetylation enhances ParB-DNA interactions affecting chromosome segregation in Streptomyces coelicolor.去乙酰化增强 ParB-DNA 相互作用，影响链霉菌的染色体分离。
Nucleic Acids Res. 2020 May 21;48(9):4902-4914. doi: 10.1093/nar/gkaa245.
本文引用的文献
1
The structure and function of bacterial actin homologs.细菌肌动蛋白同源物的结构与功能。
Cold Spring Harb Perspect Biol. 2010 Sep;2(9):a000364. doi: 10.1101/cshperspect.a000364. Epub 2010 Jul 14.
2
Membrane-associated DNA transport machines.膜相关 DNA 转运机器。
Cold Spring Harb Perspect Biol. 2010 Jul;2(7):a000406. doi: 10.1101/cshperspect.a000406. Epub 2010 Jun 23.
3
Negative membrane curvature as a cue for subcellular localization of a bacterial protein.负膜曲率作为一种细菌蛋白亚细胞定位的线索。
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13541-5. doi: 10.1073/pnas.0906851106. Epub 2009 Jul 28.
4
Localisation of DivIVA by targeting to negatively curved membranes.通过靶向负曲率膜定位 DivIVA。
EMBO J. 2009 Aug 5;28(15):2272-82. doi: 10.1038/emboj.2009.129. Epub 2009 May 28.
5
Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation.ParB-parS介导的SMC招募可组织起始区域并促进高效的染色体分离。
Cell. 2009 May 15;137(4):697-707. doi: 10.1016/j.cell.2009.04.044.
6
Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.ParB/SpoOJ 将凝聚素招募至复制起始区域可促进枯草芽孢杆菌中的染色体分离。
Cell. 2009 May 15;137(4):685-96. doi: 10.1016/j.cell.2009.02.035.
7
Growth conditions regulate the requirements for Caulobacter chromosome segregation.生长条件调节柄杆菌染色体分离的需求。
J Bacteriol. 2009 Feb;191(3):1097-100. doi: 10.1128/JB.00862-08. Epub 2008 Nov 21.
8
Non-random segregation of sister chromosomes in Escherichia coli.大肠杆菌中姐妹染色体的非随机分离。
Nature. 2008 Oct 30;455(7217):1248-50. doi: 10.1038/nature07282.
9
Dynamic control of the DNA replication initiation protein DnaA by Soj/ParA.由Soj/ParA对DNA复制起始蛋白DnaA进行动态调控。
Cell. 2008 Oct 3;135(1):74-84. doi: 10.1016/j.cell.2008.07.044.
10
F plasmid partition depends on interaction of SopA with non-specific DNA.F质粒分配依赖于SopA与非特异性DNA的相互作用。
Mol Microbiol. 2008 Nov;70(4):1000-11. doi: 10.1111/j.1365-2958.2008.06465.x. Epub 2008 Sep 30.
Zotero 插件