Wichelhaus T A, Schäfer V, Hunfeld K P, Reimer K, Fleischer W, Brade V
Institut für Medizinische Mikrobiologie, Johann Wolfgang Goethe Universität Frankfurt am Main.
Zentralbl Hyg Umweltmed. 1998 Feb;200(5-6):435-42.
Antiseptics that are locally applicable gain in significance due to their reliable microbicidal effectiveness and especially due to the rising incidence of highly resistant bacteria. This is because systemically applied antibiotics are not sufficient in eradicating superficial mucodermal bacteria and locally applied antibiotics can cause new resistance rapidly. The aim of this study was to demonstrate the microbicidal effectiveness of Poly(1-vinyl-2-pyrrodlidon)-iodine (PVP-iodine) against ten genotypical different methicillin resistant Staphylococcus aureus-(MRSA-) and five Enterococcus faecium-strains in a quantitative suspension test. The effectiveness of PVP-iodine with protein load (0.2% and 2% albumin) was tested against three MRSA strains. Without any protein load best microbicidial activity (KRt-value > 5) was obtained with concentrations in the range between 1-10% of the original Betaisodona solution after 30s exposure time. With protein load (0.2% albumin) the optimum in microbicidal effectiveness shifts to concentrations > or = 10% Betaisodona solution referring to an exposure time of 30s. With a protein load up to 2% albumin and an exposure time of 30s the bactericidal activity of the undiluted Betaisodona solution is already satisfying, while the 10% solution is not active till an exposure time of 5 min. Summing up PVP-iodine is recommended as a local mucodermal antiseptic against highly resistant gram positives.
由于具有可靠的杀菌效果，尤其是鉴于高耐药性细菌的发病率不断上升，局部适用的防腐剂变得愈发重要。这是因为全身应用抗生素不足以根除浅表粘膜皮肤细菌，而局部应用抗生素会迅速产生新的耐药性。本研究的目的是在定量悬液试验中证明聚（1-乙烯基-2-吡咯烷酮）碘（PVP-碘）对十种基因型不同的耐甲氧西林金黄色葡萄球菌（MRSA）和五种粪肠球菌菌株的杀菌效果。测试了含蛋白质负载（0.2%和2%白蛋白）的PVP-碘对三种MRSA菌株的效果。在无任何蛋白质负载的情况下，暴露30秒后，原始贝他索溶液浓度在1-10%范围内可获得最佳杀菌活性（KRt值>5）。对于含蛋白质负载（0.2%白蛋白）的情况，杀菌效果最佳时的浓度转变为≥10%贝他索溶液，暴露时间为30秒。当蛋白质负载高达2%白蛋白且暴露时间为30秒时，未稀释的贝他索溶液的杀菌活性已令人满意，而10%溶液直到暴露5分钟才有活性。总之，推荐使用PVP-碘作为针对高耐药革兰氏阳性菌的局部粘膜皮肤防腐剂。
