Xu Y, Appay M D, Heudes D, Lemoine R, Hinglais N, Michel J B, Bariéty J
INSERM Unité 430, Hôpital Broussais, Paris, France.
Virchows Arch. 1998 Mar;432(3):267-77. doi: 10.1007/s004280050165.
In the first 6 days of hypertension, infiltrated mononuclear cells were colocalized with collagen (I) mRNA-overexpressing fibroblasts in the adventitial area of unclipped kidney. The number of adventitial infiltrated mononuclear cells was correlated with adventitial collagen (I) surface expansion. After 22 days of hypertension no collagen (I) mRNA-overexpressing fibroblasts or any increase in collagen area or mononuclear cell infiltration was observed. In the interstitium of unclipped kidney, collagen (I) mRNA overexpression, collagen (I) expansion and mononuclear cell infiltration began later, from the 7th day of hypertension, and kept increasing. In the clipped kidney, after expansion in the first 6 days of hypertension, the adventitial collagen remained stable. These results suggest that in the unclipped kidney fibroblastic activation begins within the first 6 days of hypertension in the adventitial area, but is transient, and fibrosis then spreads in the interstitium. Mononuclear cell infiltration is colocalized and correlated with adventitial and interstitial fibrosis. In the first 6 days, hypertension is not the only cause of fibrosis; the same level of adventitial fibrosis is detected in the nonhypertensive clipped kidney. All observed pathological phenomena could be detected within the first 3 days of hypertension.
在高血压形成的最初6天，浸润的单核细胞与未夹闭肾脏外膜区域中I型胶原mRNA过表达的成纤维细胞共定位。外膜浸润单核细胞的数量与外膜I型胶原的面积扩张相关。高血压22天后，未观察到I型胶原mRNA过表达的成纤维细胞，也未观察到胶原面积增加或单核细胞浸润增加。在未夹闭肾脏的间质中，I型胶原mRNA过表达、I型胶原扩张和单核细胞浸润从高血压第7天开始出现得较晚，并持续增加。在夹闭的肾脏中，高血压最初6天外膜胶原扩张后保持稳定。这些结果表明，在未夹闭的肾脏中，成纤维细胞激活在高血压最初6天在外膜区域开始，但具有短暂性，随后纤维化在间质中扩散。单核细胞浸润与外膜和间质纤维化共定位并相关。在最初6天，高血压不是纤维化的唯一原因；在非高血压夹闭肾脏中检测到相同程度的外膜纤维化。所有观察到的病理现象在高血压最初3天内即可检测到。
