Moraes M F, Garcia-Cairasco N
Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1997 Nov;30(11):1319-24. doi: 10.1590/s0100-879x1997001100010.
Current methods for recording field potentials with tungsten electrodes make it virtually impossible to use the same recording electrode also as a lesioning electrode, for example for histological confirmation of the recorded site, because the lesioning procedure usually wears off the tungsten tip. Therefore, the electrode would have to be replaced after each lesioning procedure, which is a very high cost solution to the problem. We present here a low cost, easy to make, high quality glass pipette-carbon fiber microelectrode that shows resistive, signal/noise and electrochemical coupling advantages over tungsten electrodes. Also, currently used carbon fiber microelectrodes often show problems with electrical continuity, especially regarding electrochemical applications using a carbon-powder/resin mixture, with consequent low performance, besides the inconvenience of handling such a mixture. We propose here a new method for manufacturing glass pipette-carbon fiber microelectrodes with several advantages when recording intracerebral field potentials.
目前使用钨电极记录场电位的方法几乎不可能将同一个记录电极同时用作损伤电极,例如用于对记录部位进行组织学确认,因为损伤过程通常会磨损钨电极尖端。因此,每次损伤操作后都必须更换电极,这是一种成本非常高的解决办法。我们在此展示一种低成本、易于制作、高质量的玻璃微吸管 - 碳纤维微电极,它在电阻、信号/噪声和电化学耦合方面比钨电极具有优势。此外,目前使用的碳纤维微电极常常存在电连续性问题,特别是在使用碳粉/树脂混合物进行电化学应用时,随之而来的是性能低下,而且处理这种混合物也不方便。我们在此提出一种制造玻璃微吸管 - 碳纤维微电极的新方法,该方法在记录脑内场电位时具有多个优点。