Dzhanga A A
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Genetika. 1998 Jan;34(1):45-51.
A 3279-bp fragment of the Bacillus subtilis chromosome was cloned. This fragment completely restored exonuclease I activity in cells of the Escherichia coli Jm105 strain, which contained the sbcB mutation, and suppressed repair damage in cells of this mutant. The cloned fragment fully complemented a mutation that decreased exonuclease I activity in Bac. subtilis KU647 and KU1020 strains leading to the restoration of enzymatic activity and mutant phenotype suppression. The nucleotide sequence of the Bac. subtilis gene encoding the structure of exonuclease I was determined. The amino-acid sequence of this enzyme proved to have 29.7% homology with the amino-acid sequence of E. coli exonuclease I.
克隆了枯草芽孢杆菌染色体的一个3279碱基对的片段。该片段完全恢复了含有sbcB突变的大肠杆菌Jm105菌株细胞中的核酸外切酶I活性，并抑制了该突变体细胞中的修复损伤。克隆片段完全互补了枯草芽孢杆菌KU647和KU1020菌株中降低核酸外切酶I活性的突变，导致酶活性恢复和突变体表型抑制。确定了编码核酸外切酶I结构的枯草芽孢杆菌基因的核苷酸序列。该酶的氨基酸序列与大肠杆菌核酸外切酶I的氨基酸序列具有29.7%的同源性。
