[含有枯草芽孢杆菌染色体插入片段的质粒pBR 322对大肠杆菌rec B rec C突变的部分抑制作用]
[Partial suppression of rec B rec C mutations in E. coli by plasmid pBR 322 containing a Bacillus subtilis chromosome insertion].
作者信息
Barabanshchikov B I, Bashkirov V I, Gizatullin F Sh, Gulitashvili V Sh
出版信息
Tsitol Genet. 1985 Mar-Apr;19(2):128-32.
PMID:2988161
Abstract
ATP-dependent DNAase genes of Bac. subtilis were originally cloned in E. coli plasmid pBR322. These genes are expressed in rec BC mutant E. coli cells leading to a complete recovery of the enzyme activity. Bac. subtilis enzyme suppresses reparative properties of the rec BC mutant to a considerable extent but is unable to replace functionally the E. coli mutant enzyme in recombination process.
摘要
枯草芽孢杆菌的ATP依赖型DNA酶基因最初是克隆于大肠杆菌质粒pBR322中。这些基因在rec BC突变型大肠杆菌细胞中表达，可使酶活性完全恢复。枯草芽孢杆菌的酶在很大程度上抑制了rec BC突变体的修复特性，但在重组过程中无法在功能上替代大肠杆菌突变酶。
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