Evnin D N, Tarkhanova I A, Kul'berg A Ia
Biull Eksp Biol Med. 1976 Mar;81(3):328-30.
As demonstrated by immunochemical methods, the serum of partially hepatectomized rabbits contained a Fab-like fragment of IgG. This fragment was isolated by fractionation of the serum on CM-cellulose following affinity chromatography on Sepharose coated with antibodies against rabbit IgG. The sedimentation constant of the Fab-like fragment was 5.2 S; complete antigenic identity to pepsin F (ab')2 fragment was thus demonstrated. No Fab-like fragment was shown in the serum of sham-operated animals, or in the serum of partially hepatectomized rabbits injected with protease inhibitor Trasilol before and after the operation. It was concluded that Fab-like fragment found in the partially hepatectomized rabbits appeared as a result of splitting the autologous IgG with protease(s) from cells of the liver injured during the operation.
免疫化学方法证明，部分肝切除的兔子血清中含有IgG的Fab样片段。通过在包被有抗兔IgG抗体的琼脂糖凝胶上进行亲和层析后，再在CM-纤维素上对血清进行分级分离，从而分离出该片段。该Fab样片段的沉降常数为5.2S；由此证明其与胃蛋白酶F(ab')2片段具有完全的抗原同一性。在假手术动物的血清中，或在术前和术后注射蛋白酶抑制剂抑肽酶的部分肝切除兔子的血清中，均未显示出Fab样片段。得出的结论是，部分肝切除兔子中发现的Fab样片段是由于手术过程中受损肝脏细胞中的蛋白酶裂解自体IgG所致。
