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[通过计算从毛细管迁移的细胞来确定对抗原的超敏反应]

[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].

作者信息

Novikov D K, Adamenko G P, Novikova V I

出版信息

Biull Eksp Biol Med. 1976 Jun;81(6):707-10.

PMID:953309
Abstract

The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.

摘要

描述了在存在特异性抗原的情况下抑制白细胞和巨噬细胞从毛细血管迁移的测试技术。该方法高度灵敏,允许使用少量细胞和许多(多达600个及以上)毛细血管。叠氮化钠、植物血凝素和4℃孵育抑制了细胞从毛细血管的迁移。死细胞不会从毛细血管进入培养基。结核菌素和卡介苗疫苗抑制了用卡介苗免疫的豚鼠巨噬细胞的迁移,但对用葡萄球菌变应原免疫的豚鼠巨噬细胞迁移没有抑制作用。

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