Gohin I, Olivier M, Lantier I, Pépin M, Lantier F
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, Centre de Tours, Nouzilly, France.
Vet Immunol Immunopathol. 1997 Dec 12;60(1-2):111-30. doi: 10.1016/s0165-2427(97)00090-1.
The efferent lymph duct of the ovine prescapular lymph node was cannulated, and Salmonella abortusovis (SAO), a specific pathogen for sheep inducing abortion and mortality of newborn lambs, was inoculated by the subcutaneous route in this lymph node drained area. While the prescapular lymph node draining the inoculation site represented an efficient barrier for the vaccinal SAO Rv6 strain spreading, SAO 15/5 virulent bacteria were steadily detected in efferent lymph of infected sheep. The inoculation of the virulent strain of SAO induced a greater increase of the cell output than did the attenuated vaccinal strain, but proportions of blast cells appearing in the efferent lymph were similar in both cases. Flow cytometry analysis showed that B and T cell outputs were both increased during SAO infections, but while T cell subset proportions slightly decreased, B cell percentages significantly rose, and, at the peak response, almost all of the lymphoblast cells were activated B cells. Typical antibody profiles characteristic of a primary immune response were observed, and antibody titres were greater in the efferent lymph of animals inoculated with the virulent strain of SAO. Many of the cytokine mRNAs we investigated were steadily detected by RT-PCR in efferent lymph cells of control sheep, but frequencies of detection of IL-2, IFN gamma, IL-1 beta and TNF alpha mRNAs were augmented in efferent lymph cells following inoculation of both SAO virulent or vaccinal strains. IL-10 and IL-8 mRNAs could only be detected after a SAO inoculation, while detection of IL-4 mRNAs was increased only in efferent lymph cells from SAO virulent strain-infected sheep. The efferent lymph cannulation technique thus appeared a very powerful way to study the in vivo development of the immune response to SAO, in its natural host, the sheep.
将绵羊肩胛前淋巴结的输出淋巴管进行插管，并通过皮下途径在该淋巴结引流区域接种流产嗜眠沙门氏菌（SAO），SAO是一种可导致绵羊流产和新生羔羊死亡的特异性病原体。虽然引流接种部位的肩胛前淋巴结对疫苗SAO Rv6菌株的传播构成了有效屏障，但在感染绵羊的输出淋巴中仍能稳定检测到SAO 15/5毒力菌株。接种SAO毒力菌株比接种减毒疫苗菌株诱导的细胞输出增加幅度更大，但两种情况下输出淋巴中出现的母细胞比例相似。流式细胞术分析表明，SAO感染期间B细胞和T细胞的输出均增加，但T细胞亚群比例略有下降，B细胞百分比显著上升，在反应高峰期，几乎所有的淋巴母细胞都是活化的B细胞。观察到了典型的初级免疫反应抗体谱，接种SAO毒力菌株的动物输出淋巴中的抗体滴度更高。我们研究的许多细胞因子mRNA在对照绵羊的输出淋巴细胞中通过RT-PCR能稳定检测到，但接种SAO毒力菌株或疫苗菌株后，输出淋巴细胞中IL-2、IFNγ、IL-1β和TNFα mRNA的检测频率增加。IL-10和IL-8 mRNA仅在接种SAO后才能检测到，而IL-4 mRNA的检测仅在感染SAO毒力菌株的绵羊的输出淋巴细胞中增加。因此，输出淋巴插管技术似乎是研究绵羊这一自然宿主对SAO免疫反应体内发展的一种非常有效的方法。
