Yirrell D L, Reid H W, Norval M, Entrican G, Miller H R
Department of Bacteriology, University of Edinburgh, Medical School, UK.
Vet Immunol Immunopathol. 1991 Jul;28(3-4):219-35. doi: 10.1016/0165-2427(91)90116-t.
Functional and phenotypic changes in the cell populations were monitored in the popliteal efferent lymph of sheep following experimental epidermal infection with orf virus. In another group of sheep, cells from the popliteal lymph node draining the site of infection were similarly monitored and compared with the cells from contralateral popliteal and mesenteric lymph nodes. All sheep showed serological evidence of previous exposure to orf virus. Following infection, anti-orf antibody titres rose and efferent lymphocyte and blast cell output increased. Interferon-like activity was detected in efferent lymph early after orf virus but not mock infection. Lymphocytes from the draining popliteal lymph node showed antigen-specific lymphoproliferation on Days 3-7 while cells in the efferent lymph demonstrated proliferative activity on Days 4-6. The requirement for exogenous antigen-presenting cells in the culture of efferent lymphocytes varied between individual sheep. The culture supernatant from proliferating lymph node cells contained interferon-like activity but no anti-orf antibodies, the reverse of that from cultured efferent lymphocytes, perhaps indicating a different reactive T cell population. During the course of the experiment there was an increase in the percentage of efferent lymphocytes expressing MHC Class II antigens and surface immunoglobulins, the latter being recorded as a double peak. The short-term nature of the local T cell response may in part explain the incompleteness of immunity to orf virus in sheep.
在用羊口疮病毒进行实验性表皮感染后，对绵羊腘窝输出淋巴液中的细胞群体功能和表型变化进行了监测。在另一组绵羊中，对引流感染部位的腘窝淋巴结中的细胞进行了类似的监测，并与对侧腘窝和肠系膜淋巴结中的细胞进行了比较。所有绵羊均显示有先前接触过羊口疮病毒的血清学证据。感染后，抗羊口疮抗体滴度升高，输出淋巴细胞和母细胞产量增加。在羊口疮病毒感染后早期，但在模拟感染后未在输出淋巴液中检测到干扰素样活性。引流腘窝淋巴结中的淋巴细胞在第3 - 7天表现出抗原特异性淋巴细胞增殖，而输出淋巴液中的细胞在第4 - 6天表现出增殖活性。在不同的绵羊个体中，输出淋巴细胞培养对外源抗原呈递细胞的需求有所不同。增殖的淋巴结细胞培养上清液含有干扰素样活性，但不含抗羊口疮抗体，这与培养的输出淋巴细胞的情况相反，这可能表明存在不同的反应性T细胞群体。在实验过程中，表达MHC II类抗原和表面免疫球蛋白的输出淋巴细胞百分比增加，后者呈现双峰记录。局部T细胞反应的短期性质可能部分解释了绵羊对羊口疮病毒免疫的不完整性。
