Josefsen T D, Landsverk T
Department of Arctic Veterinary Medicine, Norwegian College of Veterinary Medicine, Tromsø, Norway.
Vet Immunol Immunopathol. 1997 Dec 12;60(1-2):197-202. doi: 10.1016/s0165-2427(97)00130-x.
Leukocytes in the forestomach mucosa of reindeer (Rangifer tarandus tarandus L.) were investigated by immunoperoxidase staining of cryostat sections, using monoclonal antibodies against antigens on sheep leukocytes. Mucosal samples from three free-ranging reindeer calves were compared with samples from three calves fed baled grass silage previously shown to induce increased frequency of lesions in the ruminal epithelium. In both groups, MHC-II + cells and gamma delta T cells were observed, located within or just below the basal layer of the stratified epithelium. Computer-assisted morphometric analysis showed that the number of gamma delta T cells in the ruminal mucosa was higher in the silage-fed than in the free-ranging animals. No marked difference in number of MHC-II + Langerhans cells was observed between the groups.
利用抗绵羊白细胞抗原的单克隆抗体，通过对冷冻切片进行免疫过氧化物酶染色，研究了驯鹿（Rangifer tarandus tarandus L.）前胃黏膜中的白细胞。将三只自由放养的驯鹿幼崽的黏膜样本与三只此前食用捆包青贮牧草的幼崽的样本进行了比较，此前研究表明，食用捆包青贮牧草会导致瘤胃上皮病变频率增加。在两组中均观察到MHC-II +细胞和γδ T细胞，它们位于复层上皮基底层内或基底层下方。计算机辅助形态计量分析表明，青贮饲料喂养的动物瘤胃黏膜中γδ T细胞的数量高于自由放养的动物。两组之间MHC-II +朗格汉斯细胞的数量没有明显差异。
