Min X, Xiaohui Z, Zhaixiang D, Ming O
Department of Medicine, University of Western Australia, Perth.
J Ethnopharmacol. 1998 Feb;60(1):43-51. doi: 10.1016/s0378-8741(97)00135-9.
New Zealand White female rabbits were randomly divided into three groups, each contained six rabbits, i.e. thyroidectomized and untreated rabbits (group 1), thyroidectomized rabbits treated by the Yang tonifying herbs (group 2) and sham thyroidectomized rabbits as controls (group 3). The myocardial beta-adrenoceptor density (Bmax) and affinity (Kd) of each group of rabbits were determined by radioligand binding assay technique on the thirtieth postoperative day and the data were handled by using a computer program of the Woolf plot with weight regression. Moreover, the serum levels of thyroxine (T4) and triiodothyronine (T3) of each group of rabbits were measured by radioimmunoassay technique and their heart rates (HR) were also recorded on the preoperative and thirtieth postoperative day. The results showed that the Bmax, T4, T3 and HR in group 1 were lower significantly than that in group 3 (P < 0.01-0.001), but the change of Kd in group 1 was not significant; the deviation of the indices from the normal value in group 2 was less remarkably than in group 1 other than T4.
新西兰雌性白兔被随机分为三组，每组六只，即甲状腺切除未治疗组（第1组）、甲状腺切除后用补阳药治疗组（第2组）和假手术甲状腺切除组作为对照组（第3组）。在术后第30天，采用放射性配体结合分析技术测定每组家兔心肌β-肾上腺素能受体密度（Bmax）和亲和力（Kd），并使用加权回归的Woolf图计算机程序处理数据。此外，采用放射免疫分析技术测定每组家兔血清甲状腺素（T4）和三碘甲状腺原氨酸（T3）水平，并在术前和术后第30天记录其心率（HR）。结果显示，第1组的Bmax、T4、T3和HR显著低于第3组（P<0.01-0.001），但第1组Kd的变化不显著；除T4外，第2组各指标偏离正常值的程度比第1组小。
