Kronin V, Vremec D, Shortman K
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Int Immunol. 1998 Feb;10(2):237-40. doi: 10.1093/intimm/10.2.237.
The IL-2 receptor (IL-2R) alpha chain (CD25), but not the IL-2R beta chain, is induced on dendritic cells (DC) by brief periods of culture. To test if this IL-2R alpha is important for DC function, DC were isolated from the spleens of mutant mice with the IL-2R alpha gene disrupted and compared with normal DC for ability to stimulate proliferation of allogeneic CD4 and CD8 T cells in culture. The IL-2R alpha null DC and the normal DC produced nearly identical proliferative responses from CD4 and from CD8 T cells. When the CD8 alpha+ and CD8 alpha- subsets of the IL-2R alpha null DC were separated, they also produced proliferative responses similar to that of their normal DC counterparts. Overall there was no evidence that the inducible IL-2R alpha on DC was required for DC development, for stimulation of T cells or for regulation of T cell responses.
通过短时间培养，可在树突状细胞（DC）上诱导出白细胞介素-2受体（IL-2R）α链（CD25），但不能诱导出IL-2Rβ链。为了检测这种IL-2Rα对DC功能是否重要，从IL-2Rα基因缺失的突变小鼠脾脏中分离出DC，并与正常DC比较在培养中刺激同种异体CD4和CD8 T细胞增殖的能力。IL-2Rα基因缺失的DC和正常DC对CD4和CD8 T细胞产生的增殖反应几乎相同。当将IL-2Rα基因缺失的DC的CD8α+和CD8α-亚群分离时，它们产生的增殖反应也与其正常DC对应物相似。总体而言，没有证据表明DC上可诱导的IL-2Rα对DC发育、T细胞刺激或T细胞反应调节是必需的。
