You X, Schinazi R F, Arrowood M J, Lejkowski M, Juodawlkis A S, Mead J R
Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
J Antimicrob Chemother. 1998 Feb;41(2):293-6. doi: 10.1093/jac/41.2.293.
Using a chemiluminescence immunoassay, paromomycin and lasalocid were shown to inhibit Cryptosporidium parvum growth in Madin-Darby canine kidney cells in a concentration-dependent manner. The median effective concentrations (EC50s) for paromomycin and lasalocid were 1184 mg/L and 0.4 mg/L, respectively. Neither drug was cytotoxic to host cells at concentrations up to five times their EC50s. Drug combination studies were conducted and the resulting data were analysed by the median-effect principle and combination index method. Statistically significant synergy was observed when combinations of paromomycin and lasalocid were used at ratios of 5000:1 and 2500:1. A possible mechanism for synergy is discussed.
