Hollyfield J G, Rayborn M E, Tammi M, Tammi R
Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Exp Eye Res. 1998 Feb;66(2):241-8. doi: 10.1006/exer.1997.0422.
Hyaluronan (HA) distribution in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in human, bovine, guinea pig, dog, rat and mouse tissues using a specific probe for HA (bHABC, biotinylated hyaluronan binding complex). The sclera, some regions of the choroid and vitreous body was positive for HA, as was the basal lamina of the retina (inner limiting membrane). bHABC binding was detected in the IPM of all species studied except the mouse. Predigestion with Streptomyces hyaluronidase for 3 hr before bHABC application eliminated binding in the vitreous, choroid, sclera and basal lamina of the retina, but did not eliminate bHABC binding in the IPM. In tissues from all species studied, incubation for 6 hr with hyaluronidase eliminated bHABC binding in the IPM, except for two human samples. In these two human samples, HA specific binding in the IPM persisted even after 24 hr enzyme treatment. bHABC failed to bind to any tissue layer when bHABC was preincubated with hyaluronan oligosaccharides before application. The resistance of the IPM HA to hyaluronidase digestion may reflect extensive coverage of HA binding sites by ligands present in this compartment which hinder enzyme access. The absence of bHABC binding to the IPM when the probe is preincubated with HA oligosaccharides indicates that the binding reflects specific interaction with HA. We conclude that, with the exception of the mouse, HA is a prominent constituent of the IPM, where it may serve to organize the matrix by functioning as a basic scaffold to which other macromolecules in the insoluble IPM are attached.
利用透明质酸（HA）特异性探针（生物素化透明质酸结合复合物，bHABC），对人、牛、豚鼠、狗、大鼠和小鼠组织中，从玻璃体穿过巩膜的眼后壁HA分布情况进行了评估，特别关注其在视网膜和光感受器间基质（IPM）中的定位。巩膜、脉络膜的一些区域和玻璃体对HA呈阳性反应，视网膜的基底膜（内界膜）也是如此。除小鼠外，在所研究的所有物种的IPM中均检测到bHABC结合。在应用bHABC之前，用链霉菌透明质酸酶预消化3小时可消除玻璃体、脉络膜、巩膜和视网膜基底膜中的结合，但不能消除IPM中的bHABC结合。在所有研究物种的组织中，用透明质酸酶孵育6小时可消除IPM中的bHABC结合，但有两个人类样本除外。在这两个人类样本中，即使经过24小时的酶处理，IPM中HA特异性结合仍然存在。在应用之前，将bHABC与透明质酸寡糖预孵育时，bHABC未能与任何组织层结合。IPM中HA对透明质酸酶消化的抗性可能反映了该区域存在的配体对HA结合位点的广泛覆盖，从而阻碍了酶的作用。当探针与HA寡糖预孵育时，bHABC未与IPM结合，这表明该结合反映了与HA的特异性相互作用。我们得出结论，除小鼠外，HA是IPM的主要成分，在IPM中它可能作为一个基本支架，其他不溶性IPM中的大分子附着其上，从而起到组织基质的作用。
