Hollyfield J G, Rayborn M E, Tammi R
The Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Exp Eye Res. 1997 Nov;65(5):603-8. doi: 10.1006/exer.1997.0369.
The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body was positive for HA, as was Bruch's membrane, expansive areas within the choroid, sclera and perimysial connective tissue of extraocular muscle. No HA-staining was detected in the IPM or in any other retina layer except for the basal lamina (inner limiting membrane of the retina) which abuts the vitreous. Predigestion of sections with trypsin or chondroitinase ABC before bHABC application did not produce additional HA-staining in the retina or IPM.
使用透明质酸特异性探针（生物素化透明质酸结合复合物，bHABC）对小鼠组织进行评估，以研究透明质酸（HA）在眼后极壁从玻璃体穿过巩膜的分布情况，特别关注其在视网膜和光感受器间基质（IPM）中的定位。玻璃体、布鲁赫膜、脉络膜内的扩张区域、巩膜以及眼外肌的肌周结缔组织均呈HA阳性。除了紧邻玻璃体的基膜（视网膜内界膜）外，在IPM或视网膜的任何其他层均未检测到HA染色。在用bHABC处理之前，用胰蛋白酶或软骨素酶ABC对切片进行预消化，并未在视网膜或IPM中产生额外的HA染色。
