Supersac G, Piémont Y, Kubina M, Prévost G, Foster T J
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur, 3, rue Koeberlé, Strasbourg, F-67000, France.
Microb Pathog. 1998 Apr;24(4):241-51. doi: 10.1006/mpat.1997.0192.
Purified gamma-toxin is known to have a proinflammatory effect in the rabbit vitreous humor. To assess the biological role of the gamma-toxin, when expressed in vivo by Staphylococcus aureus strain Newman, the vitreous humor of rabbit eye was used as an infection model. A gamma-toxin-deficient mutant of strain Newman was constructed by allelic replacement. S. aureus Newman wild-type, its hlg-deficient derivative strain (N65) and the strain N65 complemented with the wild-type hlg+ gene were injected into the vitreous humor of rabbit eye. All three strains produced a strong proinflammatory effect in the eye conjunctiva, posterior and anterior chambers, suggesting a role for another unidentified proinflammatory component of strain Newman distinct from the gamma-toxin. These components are not the leucocidin of Panton-Valentine, beta-toxin or alpha-toxin which are not produced by this strain. Only the hlg-deficient mutant lacked the ability to cause inflammation in the eyelid, whereas the two Hlg-producing strains gave strong inflammation. These data suggest that in vivo, strain Newman produces as yet unidentified proinflammatory molecules and that the in vivo-produced HlgA, HlgB and HlgC molecules expressed by the gamma-toxin locus, contribute in part to the inflammatory process observed in vivo in the rabbit eye.
已知纯化的γ毒素在兔玻璃体液中具有促炎作用。为了评估γ毒素在金黄色葡萄球菌纽曼菌株体内表达时的生物学作用，将兔眼玻璃体液用作感染模型。通过等位基因置换构建了纽曼菌株的γ毒素缺陷突变体。将金黄色葡萄球菌纽曼野生型、其hlg缺陷衍生菌株（N65）以及用野生型hlg +基因互补的N65菌株注入兔眼玻璃体液中。所有这三种菌株在眼结膜、后房和前房均产生强烈的促炎作用，这表明纽曼菌株中存在另一种不同于γ毒素的未鉴定促炎成分。这些成分不是该菌株不产生的潘顿-瓦伦丁杀白细胞素、β毒素或α毒素。只有hlg缺陷突变体缺乏引起眼睑炎症的能力，而两种产生Hlg的菌株则引起强烈炎症。这些数据表明，在体内，纽曼菌株产生尚未鉴定的促炎分子，并且由γ毒素基因座表达的体内产生的HlgA、HlgB和HlgC分子部分促成了兔眼中观察到的体内炎症过程。
