Viallet J P, Prin F, Olivera-Martinez I, Hirsinger E, Pourquié O, Dhouailly D
Equipe Biologie de la Différenciation Epithéliale, UMR CNRS 5538, Laboratoire d'Etude de la Différenciation et de l'Adhérence Cellulaires, Institut Albert Bonniot, Université Joseph Fourier, Grenoble, France.
Mech Dev. 1998 Mar;72(1-2):159-68. doi: 10.1016/s0925-4773(98)00027-6.
The chick dermis is known to control the formation of feathers and interfeathery skin in a hexagonal pattern. The evidence that the segregation of two types of fibroblasts involves Delta/Notch signalling is based on three facts. Rings of C-Delta-1-expressing fibroblasts precede and delimit the forming feather primordia. C-Delta-1 is uniformly expressed in the dermis of the scaleless mutant, which is almost entirely devoid of feathers. Feather development is inhibited by overexpression of C-Delta-1 in wild type dermis using a retroviral construct. We also show that the distribution of C-Delta-1 in the mutant dermis can be rescued by its association with a wild type epidermis, which acts as a permissive inducer, or by epidermal secreted proteins like FGF2.
已知鸡的真皮能以六边形模式控制羽毛和羽毛间皮肤的形成。两种成纤维细胞的分离涉及Delta/Notch信号传导这一证据基于三个事实。表达C-Delta-1的成纤维细胞环先于并界定正在形成的羽毛原基。C-Delta-1在无鳞突变体的真皮中均匀表达，该突变体几乎完全没有羽毛。使用逆转录病毒构建体在野生型真皮中过表达C-Delta-1可抑制羽毛发育。我们还表明，突变体真皮中C-Delta-1的分布可通过与作为许可诱导剂的野生型表皮结合，或通过FGF2等表皮分泌蛋白来挽救。
