Dent J A, Han M
Department of Molecular, Cellular and Developmental Biology, Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, CO 80309-0347, USA.
Mech Dev. 1998 Mar;72(1-2):179-82. doi: 10.1016/s0925-4773(98)00026-4.
let-60 ras plays roles in the differentiation of several C. elegans tissues (Yochem, J., Sundaram, M., Han, M., 1997. Ras is required for a limited number of cell fates and not for general proliferation in Caenorhabditis elegans. Mol. Cell. Biol. 17, 2716-2722). To understand the transcriptional regulation of ras and to identify new functions for ras in development, we examined expression patterns of let-60::lacZ and let-60::GFP reporter constructs in C. elegans hermaphrodites. Fusion constructs were expressed in vulval precursor cells, sex myoblasts and in cell lineages of the somatic gonad, germline cells (GFP construct only), hypodermis, muscle and nervous system.
let-60 ras在几种秀丽隐杆线虫组织的分化过程中发挥作用（Yochem, J., Sundaram, M., Han, M., 1997年。《秀丽隐杆线虫中Ras对于有限数量的细胞命运是必需的，而非对于一般增殖是必需的》。《分子与细胞生物学》17卷，2716 - 2722页）。为了了解ras的转录调控并确定ras在发育中的新功能，我们检测了秀丽隐杆线虫雌雄同体中let-60::lacZ和let-60::GFP报告基因构建体的表达模式。融合构建体在阴门前体细胞、性肌母细胞以及体细胞性腺、生殖细胞（仅GFP构建体）、皮下组织、肌肉和神经系统的细胞谱系中表达。
