Jayadev Ranjay, Chi Qiuyi, Sherwood David R
Department of Biology, Duke University, Durham, NC, USA.
MicroPubl Biol. 2023 Aug 25;2023. doi: 10.17912/micropub.biology.000931. eCollection 2023.
Ras GTPases regulate many developmental and physiological processes and mutations in Ras are associated with numerous human cancers. Here, we report the function, levels, and localization of an N-terminal knock-in of mNeonGreen (mNG) into LET-60 /Ras. mNG:: LET-60 interferes with some but not all LET-60 /Ras functions. mNG:: LET-60 is broadly present in tissues, found at different levels in cells, and concentrates in distinct subcellular compartments, including the nucleolus, nucleus, intracellular region, and plasma membrane. These results suggest that mNG:: LET-60 can be a useful tool for determining LET-60 levels and localization once its functionality in a developmental or physiological process is established.
Ras GTP酶调控许多发育和生理过程，Ras突变与多种人类癌症相关。在此，我们报告了在LET-60/Ras的N端敲入mNeonGreen（mNG）的功能、水平和定位。mNG::LET-60会干扰部分而非全部LET-60/Ras的功能。mNG::LET-60广泛存在于组织中，在细胞中的水平各异，并集中于不同的亚细胞区室，包括核仁、细胞核、细胞内区域和质膜。这些结果表明，一旦确定mNG::LET-60在发育或生理过程中的功能，它可能成为确定LET-60水平和定位的有用工具。
