Waterworth M W, Barbezat G O, Hickman R, Terblanche J
Br J Surg. 1976 Aug;63(8):617-20. doi: 10.1002/bjs.1800630814.
This study was designed to assess the value of glucagon infusion in pigs with experimental pancreatitis. The condition was induced by injection of bile into the pancreatic duct directly after intravenous injection of secretion. Macroscopic haemorrpagic pancreatitis ensued immediately, and was accompanied by a rise in the serum amylase of five to ten times. An experimental group of 15 pigs was given glucagon by continous infusion for 18 hours beginning 18 hours after induction of pancreatitis. A control group was given intravenous saline. Fourteen of the 15 treated animals survived for 1 week and 3 died within 2 weeks, after which the remaining animals were sacrificed. Ten of the 15 controls died within the first week. Serum amylase levels in both groups began to decline when infusion was commenced, but levels in the treated group were significantly lower than in the controls. Autopsy showed prominent peripancreatic granulation tissue in the treated animals, a significant incidence of pericarditis in both groups and pancreatic pseudocysts in 3 controls and 1 treated animal. These results suggest that glucagon effectively reduced the mortality in pigs with experimental pancreatitis and that a controlled clinical trial of treatment is justified.
本研究旨在评估胰高血糖素输注对实验性胰腺炎猪的价值。该病症是通过在静脉注射分泌液后直接将胆汁注入胰管诱导产生的。随即出现了肉眼可见的出血性胰腺炎，并伴有血清淀粉酶升高五到十倍。15只猪的实验组在胰腺炎诱导18小时后开始持续输注胰高血糖素18小时。对照组给予静脉生理盐水。15只接受治疗的动物中有14只存活了1周，3只在2周内死亡，之后其余动物被处死。15只对照组中有10只在第一周内死亡。两组的血清淀粉酶水平在开始输注时均开始下降，但治疗组的水平明显低于对照组。尸检显示，治疗组动物胰腺周围有明显的肉芽组织，两组均有显著的心包炎发生率，3只对照组动物和1只治疗组动物出现胰腺假性囊肿。这些结果表明，胰高血糖素有效地降低了实验性胰腺炎猪的死亡率，因此进行对照临床试验是合理的。
