Akiyama K, Shida T, Yasueda H, Saito A, Hasegawa M, Maeda Y, Takesako K, Yamaguchi H, Kato H
National Sagamihara Hospital, Kanagawa, Japan.
Allergy. 1998 Feb;53(2):173-9. doi: 10.1111/j.1398-9995.1998.tb03866.x.
In the present study, we assayed mannan-specific IgE and IgG antibodies in samples of serum isolated from blood collected from adult patients with bronchial asthma, using a liquid-phase method with a polysaccharide, mannan (Mn), purified from Candida albicans (C. alb), and investigated the relationships of allergenicity among a crude extract of C. alb, purified Mn, and acid protease (AP), The correlations between the titers of anti-Mn A and anti-Mn B IgE and IgG were very strong, and the levels of inhibition of anti-Mn A IgE and IgG reactions by Mn A and Mn B were almost identical. Although no common allergenicity was observed between Mn A and AP because there was no correlation between the titers of anti-Mn A and anti-AP IgE, and no inhibition of the anti-Mn A IgE reaction by AP, both antigens were found to exist in crude C. alb. The level of inhibition of anti-crude C. alb IgG reaction by Mn A or Mn B was about 60%. Approximately 70% inhibition of the anti-Mn A IgE reaction was observed for eight different fungal allergen extracts, but no inhibition was observed for 11 of the other fungal allergen extracts tested. The above results indicate that common antigenicity was observed between Mn A and Mn B in the human IgE and IgG antibody production system, and the cross-allergenicity observed among some fungi was considered to be the result of the common antigenicity of Mn isoforms.
在本研究中，我们使用一种液相方法，采用从白色念珠菌（C. alb）中纯化的多糖甘露聚糖（Mn），检测了从成年支气管哮喘患者采集的血液中分离的血清样本中的甘露聚糖特异性IgE和IgG抗体，并研究了白色念珠菌粗提物、纯化的Mn和酸性蛋白酶（AP）之间的变应原性关系。抗Mn A和抗Mn B IgE及IgG滴度之间的相关性非常强，并且Mn A和Mn B对抗Mn A IgE和IgG反应的抑制水平几乎相同。尽管由于抗Mn A和抗AP IgE滴度之间没有相关性，且AP对抗Mn A IgE反应没有抑制作用，未观察到Mn A和AP之间存在共同变应原性，但两种抗原均存在于白色念珠菌粗提物中。Mn A或Mn B对抗白色念珠菌粗提物IgG反应的抑制水平约为60%。在8种不同的真菌变应原提取物中观察到约70%的抗Mn A IgE反应受到抑制，但在测试的其他11种真菌变应原提取物中未观察到抑制作用。上述结果表明，在人类IgE和IgG抗体产生系统中，Mn A和Mn B之间存在共同抗原性，并且在一些真菌中观察到的交叉变应原性被认为是Mn异构体共同抗原性的结果。
