Kahl B, Herrmann M, Everding A S, Koch H G, Becker K, Harms E, Proctor R A, Peters G
Institute of Medical Microbiology and Department of Pediatrics, University of Muenster Hospital and Clinics, Germany.
J Infect Dis. 1998 Apr;177(4):1023-9. doi: 10.1086/515238.
In a 34-month prospective study to determine the prevalence of Staphylococcus aureus small colony variants (SCVs) in cystic fibrosis (CF) patients, S. aureus SCVs or SCVs plus normal S. aureus were recovered from 26 of 78 patients; 27 patients harbored only normal S. aureus. By pulsed-field gel electrophoresis, clonal identity was demonstrated of SCV and normal strains isolated at the same time and of multiple S. aureus SCV and normal strains in consecutive specimens from individual patients. All S. aureus SCVs were resistant to antifolate antibiotics, while the corresponding parent strains were susceptible, and in 11 of 12 SCV/normal pairs, gentamicin was less active against S. aureus with the SCV phenotype than against the normal isolate. Analysis of the underlying auxotrophism of SCVs revealed hemin, thymidine, and/or menadione dependencies. Thus, S. aureus SCVs are highly prevalent in respiratory secretions of CF patients, persist over extended periods, and may contribute to S. aureus persistence in CF patients.
在一项为期34个月的前瞻性研究中，为确定囊性纤维化（CF）患者中金黄色葡萄球菌小菌落变异株（SCV）的流行情况，从78例患者中的26例分离出了金黄色葡萄球菌SCV或SCV加正常金黄色葡萄球菌；27例患者仅携带正常金黄色葡萄球菌。通过脉冲场凝胶电泳，证明了同时分离出的SCV和正常菌株以及来自个体患者连续标本中的多个金黄色葡萄球菌SCV和正常菌株的克隆一致性。所有金黄色葡萄球菌SCV均对抗叶酸抗生素耐药，而相应的亲本菌株敏感，并且在12对SCV/正常菌株中，有11对中庆大霉素对具有SCV表型的金黄色葡萄球菌的活性低于对正常分离株的活性。对SCV潜在营养缺陷型的分析显示其对血红素、胸苷和/或甲萘醌有依赖性。因此，金黄色葡萄球菌SCV在CF患者的呼吸道分泌物中高度流行，长期持续存在，并可能导致金黄色葡萄球菌在CF患者体内持续存在。
