1型人类免疫缺陷病毒糖蛋白160和糖蛋白41与白色念珠菌的结合在体外选择性增强念珠菌的毒力。

Human immunodeficiency virus type 1 gp160 and gp41 binding to Candida albicans selectively enhances candidal virulence in vitro.

作者信息

Gruber A, Lukasser-Vogl E, Borg-von Zepelin M, Dierich M P, Würzner R

机构信息

Institut für Hygiene, University of Innsbruck, Austria.

出版信息

J Infect Dis. 1998 Apr;177(4):1057-63. doi: 10.1086/515231.

DOI:10.1086/515231

Abstract

Previously, it has been shown that human immunodeficiency virus (HIV)-1 envelope proteins gp160 and gp41 bind to Candida albicans. Whether this interaction affects candidal virulence in vitro was investigated. HIV-1 gp160 or gp120 treatment of C. albicans significantly altered neither growth nor phospholipase activity of the fungus. However, treatment of C. albicans with gp160, but not with gp120, led to an elevation of free and cell-bound aspartate proteinase. In addition, culture supernatants obtained from C. albicans treated with gp160 or gp41, but not with gp120, showed a strong increase in proteinase activity. Finally, C. albicans viable yeast cells treated with gp160 or gp41 and serum were phagocytosed by polymorphonuclear leukocytes to a lesser extent than was C. albicans treated with gp120 and serum or serum alone. These findings suggest that the interaction between HIV-1 gp160 and C. albicans may promote the virulence of C. albicans in HIV-1-positive patients.

摘要

此前已有研究表明，人类免疫缺陷病毒1型（HIV-1）包膜蛋白gp160和gp41可与白色念珠菌结合。本研究调查了这种相互作用是否会在体外影响念珠菌的毒力。用HIV-1 gp160或gp120处理白色念珠菌，对该真菌的生长和磷脂酶活性均无显著影响。然而，用gp160而非gp120处理白色念珠菌，会导致游离型和细胞结合型天冬氨酸蛋白酶水平升高。此外，用gp160或gp41而非gp120处理白色念珠菌后获得的培养上清液，其蛋白酶活性显著增强。最后，用gp160或gp41及血清处理的白色念珠菌活酵母细胞，被多形核白细胞吞噬的程度低于用gp120及血清或仅用血清处理的白色念珠菌。这些发现表明，HIV-1 gp160与白色念珠菌之间的相互作用可能会增强HIV-1阳性患者体内白色念珠菌的毒力。